Dna Technology
Mostrando 1-12 de 641 artigos, teses e dissertações.
-
1. DNA methylation, environmental exposures and early embryo development
Abstract The first crucial step in the developmental program occurs during pre-implantation, the time after the oocyte has been fertilized and before the embryo implants in the uterus. This period represents a vulnerable window as the epigenome undergoes dynamic changes in DNA methylation profiles. Alterations in the early embryonic reprogramming wave can im
Anim. Reprod.. Publicado em: 28/11/2019
-
2. Prevalence and Molecular Analysis of Novel Newly Identified Periodontal Pathogens in Subgingival Plaque Samples of Saudi Patients with Chronic Periodontitis
Abstract Objective: To evaluate the prevalence of novel newly identified periodontal pathogenic strains in subgingival plaque samples and relate it with bleeding on probing, probing pocket depth and age. Material and Methods: 268 chronic periodontitis patients with a mean age of 46.0 ± 6.0 years were included. The following microorganisms were evaluated:
Pesqui. Bras. Odontopediatria Clín. Integr.. Publicado em: 31/10/2019
-
3. Development of an Extraction Protocol for Fetal Sexing from the Fourth Week of Gestation by Real Time PCR
Abstract Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and clinical applications. This research developed a technology which allowed the extraction of fetal DNA by a non-invasive method that offers no risk to the mother or fetus. A total of 132 pregnant women participated in this inquiry. The DNA extraction was
Braz. arch. biol. technol.. Publicado em: 24/10/2019
-
4. Effect of miR-134 against myocardial hypoxia/reoxygenation injury by directly targeting NOS3 and regulating PI3K/Akt pathway
Abstract Purpose To reveal the function of miR-134 in myocardial ischemia. Methods Real-time PCR and western blotting were performed to measure the expression of miR-134, nitric oxide synthase 3 (NOS3) and apoptotic-associated proteins. Lactic dehydrogenase (LDH) assay, cell counting kit-8 (CCK-8), Hoechst 33342/PI double staining and flow cytometry assay
Acta Cir. Bras.. Publicado em: 14/10/2019
-
5. Engineering biodiversity as a model for the species conservation
Abstract: Early humans have domesticated plant and animal species based on ancient empirical concepts (Darwin 1868, 1876). In 1886, Mendel established a new paradigm of hereditary laws (Mendel 1866, 1870, 1950) based on genotypic and phenotypic traits of cross-compatible species, establishing a complex breeding technology that is currently utilized for the d
An. Acad. Bras. Ciênc.. Publicado em: 30/09/2019
-
6. DNA and the displacement of certainties in brazilian family law
Resumo Enfocando o impacto da tecnologia do DNA no direito de família brasileiro, pela análise de leis, jurisprudência e processos particulares lidando com disputas de paternidade, mapeamos tendências de mudança ao longo dos últimos trinta anos. Mostramos como, após um momento de ceticismo inicial, a evidência genética parece dominar o cenário lega
Sex., Salud Soc. (Rio J.). Publicado em: 09/09/2019
-
7. Impact of co-blocking the costimulatory signals on immune-related genes after high-risk rabbit corneal allograft using 2nd-generation DNA sequencing technology
Abstract The aim of this study was to evaluate the impact and mechanism of co-blocking of costimulatory signals CD28-B7-CD40-CD40L during immune allograft rejection. Forty-eight recipient rabbits were prepared as a high-risk corneal allograft model. After surgery, the animals were randomly divided into: control group, MR1 group, anti-B7 group, and co-blockin
Genet. Mol. Biol.. Publicado em: 18/07/2019
-
8. Edição genética: riscos e benefícios da modificação do DNA humano
Resumo O artigo analisa discussões sobre edição genética humana encontradas em artigos científicos, declarações institucionais e proferidas no International Summit on Gene Editing realizado em 2015. Objetiva-se explicitar e refletir sobre argumentos favoráveis e contrários à modificação do DNA. A edição genética pode desenvolver novas terapêu
Rev. Bioét.. Publicado em: 01/07/2019
-
9. Use of molecular techniques for the analysis of the microbiological quality of fish marketed in the municipality of Cuiabá, Mato Grosso, Brazil
ABSTRACT The present study was stimulated by the growing demand for healthy food and the technology available for the detection and identification of microbial organisms in fishery products. Molecular tools were used to identify the bacterial community and detect the presence of pathogenic species in samples of farmed and wild-caught fish. Samples of muscle
Food Sci. Technol. Publicado em: 22/10/2018
-
10. Perspectives for cancer immunotherapy mediated by p19Arf plus interferon-beta gene transfer
While cancer immunotherapy has gained much deserved attention in recent years, many areas regarding the optimization of such modalities remain unexplored, including the development of novel approaches and the strategic combination of therapies that target multiple aspects of the cancer-immunity cycle. Our own work involves the use of gene transfer technology
Clinics. Publicado em: 06/09/2018
-
11. Screening a bacterium and its effect on the biological degumming of ramie and kenaf
ABSTRACT: Biological degumming is an eco-friendly, efficient, high-quality and low-cost method that has become the leading bast fiber degumming technology. However, bacterial strains with short degumming cycles, high gum removal rates and small fiber damage are few. To screen high quality microbial resources with bast-fiber biological degumming function, soi
Sci. agric. (Piracicaba, Braz.). Publicado em: 2018-09
-
12. Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation
ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showe
Braz. arch. biol. technol.. Publicado em: 25/06/2018