Dna Hpv Probes
Mostrando 1-12 de 52 artigos, teses e dissertações.
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1. Valor da captura híbrida para o papilomavírus humano (HPV) no seguimento de pacientes submetidos à conização do colo uterino devido a lesão intraepitelial de alto grau por cirurgia de alta frequência (CAF) / The value of the HPV DNA test on the follow-up of the patients treated for high grade cervical intraepithelial lesions by conization with large loop excision of the transformation zone (LLETZ)
INTRODUÇÃO: A lesão intraepitelial cervical de alto grau causada pelo HPV, precursora do câncer cervical, é facilmente diagnosticada e seu tratamento pode ser realizado de maneira ambulatorial, sem muitas complicações. Mesmo assim, o seguimento das pacientes tratadas deve ser feito de maneira criteriosa e sistemática para que a recorrência ou persis
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/05/2012
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2. Prevalência de HPV em adolescentes virgens e com atividade sexual / Prevalence of Human Papillomavirus in virgin and sexual activity adolescents
O Papilomavírus humano (HPV) é um vírus DNA bastante prevalente em estimativas mundiais, sendo a DST isolada mais frequente no mundo. Existem poucos dados sobre prevalência em adolescentes sem vida sexual ativa, havendo ainda muitos tabus nesta faixa etária. O presente estudo tem por objetivo identificar a prevalência de Papilomavírus humano através
Publicado em: 2010
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3. Prevalence by HPV and squamous intraepithelial lesions in pregnant women / PrevalÃncia de HPH e lesÃes intraepiteliais escamosas em gestantes
This work studies the prevalence (cross sectional) on infection by HPV in uterine cervical of pregnant women evaluating the subgroups of HPV more prevalents and the association in relation to the development of squamous intraepithelial lesions. The methodology was constituted of questionary applied to 272 pregnant women independent of pregnant age and of bei
Publicado em: 2008
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4. HPV detection in oral leukoplakia and oral squamous cell carcinoma / "Detecção do HPV em leucoplasias e carcinomas epidermóides orais"
It is still highly controversial whether HPV can be considered an aetiological or risk factor for the development of malignant/premalignant lesions of the oral cavity. There is an agreement that there is at least quantitative evidence to relate HPV and oral carcinogenesis, since in general the studies find a proportional relation between degree of malignancy
Publicado em: 2007
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5. Human papillomavirus prevalence among women with cervical intraepithelial neoplasia III and invasive cervical cancer from Goiânia, Brazil
This study estimated the prevalence and distribution of human papillomavirus (HPV) types among women with cervical intraepithelial neoplasia (CIN) grade III and invasive cervical cancer from Goiás (Brazil Central Region). Seventy-four cases were analyzed and consisted of 18 CIN III, 48 squamous cell carcinomas, 4 adenocarcinomas, 1 adenosquamous carcinoma a
Memórias do Instituto Oswaldo Cruz. Publicado em: 2003-03
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6. Investigação do papilomavirus humano (HPV) por imuno-histoquimica e hibridização "in situ" em biopsias endoscopicas de esofago
Human Papillomavirus has been describe as a risk factor to benign and malign esophageallesions. The HPV s eommons in esophageal epithelium are 6, 11, 16, 18, 31, 33 and 35. We had select fourty nine endoscopie biopsies which histologic analysis had show koilocytotic atypia in different grades. We has use immunohistochemical and in situ hibridization reaction
Publicado em: 1996
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7. Use of Probemix and OmniProbe biotinylated cDNA probes for detecting HPV infection in biopsy specimens from the genital tract.
AIMS--To compare two commercially available pan probes for the identification of human papillomavirus (HPV) DNA expression in histological sections and to type the HPV positive cases. METHODS--97 formalin fixed, paraffin wax embedded biopsy specimens from the genital tract were tested for HPV positivity with in situ hybridisation using biotinylated cDNA pan
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8. Sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation.
The sensitivity of digoxigenin and biotin labelled DNA probes for the detection of human papillomavirus (HPV) by dot blotting and in situ hybridisation was compared in tissues from cervical, laryngeal, and anogenital neoplasia. Probes were either labelled with digoxigenin by the random primer technique and detected with anti-digoxigenin antibody, or labelled
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9. Isolation of a novel human papillomavirus (type 51) from a cervical condyloma.
We cloned the DNA from a novel human papillomavirus (HPV) present in a cervical condyloma. When DNA from this isolate was hybridized at high stringency with HPV types 1 through 50 (HPV-1 through HPV-50), it showed weak homology with HPV-6 and -16 and stronger homology with HPV-26. A detailed restriction endonuclease map was prepared which showed marked diffe
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10. Detection of transcripts of human papillomaviruses 16 and 18 in cancer-derived cell lines and cervical biopsies by enzyme immunoassay for DNA-RNA hybrids following solution hybridization.
mRNAs of human papillomaviruses (HPV) 16 and 18 were detected in cancer-derived cell lines and genital tract biopsy specimens by a novel hybridization assay. Biotinylated whole genomic HPV DNA probes were hybridized in solution to extracted total nucleic acids. Hybrids between the labeled probes and RNA transcripts were captured on a microplate coated with a
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11. Detection and Quantitation of Human Papillomavirus by Using the Fluorescent 5′ Exonuclease Assay
A method for the detection and quantitation of oncogenic human papillomavirus (HPV) was developed by using the fluorescent 5′ exonuclease assay. The method is based on the amplification of a 180-bp fragment from the 3′ part of the E1 open reading frame in a single PCR with type-specific probes for HPV types 16, 18, 31, 33, and 35. The probes can be used
American Society for Microbiology.
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12. Detection and typing of human papillomavirus using the Vira Type "in situ" kit: comparison with a conventional dot blot technique.
A new commercial kit (Vira Type "in situ", Life Technologies, Inc., Molecular Diagnostics Division, Guithersburg, Maryland, USA) for the detection of human papillomavirus (HPV) types 6, 11, 16, 18, 31, 33 and 35 in routinely processed human anogenital tissue was compared with a conventional dot blot assay for HPV 6, 11, 16 and 18. Both systems use double-str