Dna Forensics
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Using the technique of genetic identification: picture of reality official identification of the Brazilian and the importance of role of dentistry-on forensic team / Utilização da técnica de identificação genética: panorama da realidade dos serviços oficiais de identificação brasileiros e a importância da atuação do cirurgião-dentista na equipe forense
The analysis of DNA can be considered major technical advances for criminal investigation since the discovery of fingerprints. Its emergence has brought new paradigms for the criteria used in the formulation of culpability in the field of criminal law, as well as the establishment of legal certainty in relations of affiliation, field of civil law. It was inc
Publicado em: 2009
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2. MITOMAP: a human mitochondrial genome database—2004 update
MITOMAP (http://www.MITOMAP.org), a database for the human mitochondrial genome, has grown rapidly in data content over the past several years as interest in the role of mitochondrial DNA (mtDNA) variation in human origins, forensics, degenerative diseases, cancer and aging has increased dramatically. To accommodate this information explosion, MITOMAP has im
Oxford University Press.
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3. Whole genome amplification from a single cell: implications for genetic analysis.
We have developed an in vitro method for amplifying a large fraction of the DNA sequences present in a single haploid cell by repeated primer extensions using a mixture of 15-base random oligonucleotides. We studied 12 genetic loci and estimate that the probability of amplifying any sequence in the genome to a minimum of 30 copies is not less than 0.78 (95%
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4. Detection of specific DNA sequences by fluorescence amplification: a color complementation assay.
We have developed a color complementation assay that allows rapid screening of specific genomic DNA sequences. It is based on the simultaneous amplification of two or more DNA segments with fluorescent oligonucleotide primers such that the generation of a color, or combination of colors, can be visualized and used for diagnosis. Color complementation assay o
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5. Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.
A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell beta-globin allele in genomic DNA without additional steps of probe hybridization, ligation, or restriction enzyme cleavage. Two allele-specific
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6. Comprehensive human genome amplification using multiple displacement amplification
Fundamental to most genetic analysis is availability of genomic DNA of adequate quality and quantity. Because DNA yield from human samples is frequently limiting, much effort has been invested in developing methods for whole genome amplification (WGA) by random or degenerate oligonucleotide-primed PCR. However, existing WGA methods like degenerate oligonucle
National Academy of Sciences.
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7. Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA
Genetic analysis of limiting quantities of genomic DNA play an important role in DNA forensics, paleoarcheology, genetic disease diagnosis, genetic linkage analysis, and genetic diversity studies. We have tested the ability of degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR) to amplify picogram quantities of human genomic DNA for th
The National Academy of Sciences of the USA.
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8. Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis
Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)–PCR describes a new paradigm for primer design that renders assays as efficient as symmetric PCR assays, regardless of primer ratio
National Academy of Sciences.
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9. Genetic differences at four DNA typing loci in Finnish, Italian, and mixed Caucasian populations.
Highly polymorphic segments of the human genome containing variable numbers of tandem repeats (VNTRs) have been widely used to establish DNA profiles of individuals for use in forensics. Methods of estimating the probability of occurrence of matching DNA profiles between two randomly selected individuals have been subject to extensive debate regarding the po
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10. Teaching Cell Biology to Nonscience Majors Through Forensics, or How to Design a Killer Course
Nonscience majors often do not respond to traditional lecture-only biology courses. However, these students still need exposure to basic biological concepts. To accomplish this goal, forensic science was paired with compatible cell biology subjects. Several topics such as human development and molecular biology were found to fulfill this purpose. Another goa
The American Society for Cell Biology.