Dna Extraction
Mostrando 1-12 de 953 artigos, teses e dissertações.
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1. SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
Resumo A Organização Mundial da Saúde declarou a disseminação generalizada do SARS-CoV-2 e sua doença associada (COVID-19) uma emergência de saúde pública. O teste padrão ouro para detecção do vírus é o RT-PCR, realizado a partir de amostras de swab nasofaríngeo (NPS). No entanto, esse exame pode ser desconfortável para o paciente e requer tr
Brazilian Dental Journal. Publicado em: 2022
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2. Expression Levels of the CA9, WT1, and PRAME Genes and Genotyping-Associated Antigens for the Diagnosis and Prognosis of Colorectal Cancer
ABSTRACT Background Colorectal cancer (CRC) is the third most prevalent type of cancer worldwide, and is one of the major health problems in Asia, Africa, Europe, and America. The tumor antigens recently are of interesting indicators as diagnostic and prognostic tools, The aim of the present study is to detect the expression levels of carbonic anhydrase IX
Journal of Coloproctology (Rio de Janeiro). Publicado em: 2022
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3. Apoplexy in sporadic pituitary adenomas: a single referral center experience and AIP mutation analysis
ABSTRACT Objective: To analyze the clinical, laboratory, and radiological findings and management of patients with clinical pituitary apoplexy and to screen for aryl hydrocarbon receptor-interacting protein (AIP) mutations. Subjects and methods: The clinical findings were collected from the medical records of consecutive sporadic pituitary adenoma patients
Arch. Endocrinol. Metab.. Publicado em: 2021-06
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4. Improvement of Mycobacterium tuberculosis detection in sputum using DNA extracted by sonication
Abstract Tuberculosis (TB) is one of the infectious diseases with high mortality in the world. DNA amplification techniques have been used to overcome barriers to the diagnosis of this disease. However, the success of these methodologies is highly dependent on the DNA obtained from the sample. This study was carried out to verify whether the DNA extracted by
Braz J Infect Dis. Publicado em: 2020-10
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5. SEQUÊNCIA DIDÁTICA PARA A PROMOÇÃO DE ESTUDO PRÁTICO E MULTIDISCIPLINAR COM MATERIAIS ACESSÍVEIS
Innovation and multidisciplinarity are the way to accelerate improvements in education, as well as the search for contextualized content. The multidisciplinarity between chemistry and Biology, allows to explore various contents, one of them is the environment. Works that offer alternatives to simpler experiments are of great help professors. In this context,
Quím. Nova. Publicado em: 2020-05
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6. Antibacterial Effects of Cuminum cyminum Extract Against Enterococcus Faecalis Biofilms From Clinical Isolates
Abstract Objective: To compare the antibacterial efficacy of Cuminum cyminum (cumin) extract and 2% chlorhexidine. Material and Methods: E. faecalis was isolated from non-vital teeth with chronic apical abscess. Samples were then bred in the ChromAgar medium. Subsequently, E. faecalis bacteria’s DNA extraction was performed. DNA was then amplified by con
Pesqui. Bras. Odontopediatria Clín. Integr.. Publicado em: 13/01/2020
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7. Lower limb muscle strength and serotonin receptor gene polymorphism as factors associated in women with fibromyalgia
Abstract Background: Fibromyalgia (FM) is a chronic pain syndrome characterized by generalized skeletal muscle chronic pain. Its etiology is not well defined, because there are several factors that may trigger it such as physical and/or emotional stresses, or a genetic susceptibility, involving serotonergic, dopaminergic and catecholaminergic paths. The obj
Adv. rheumatol.. Publicado em: 13/01/2020
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8. Validation of reference genes for gene expression studies in bovine oocytes and cumulus cells derived from in vitro maturation
Abstract Quantitative real-time PCR (qPCR) is a valuable tool for gene expression studies and it is necessary to choose an ideal endogenous reference gene for data normalization. This work studied a set of reference genes in oocytes and cumulus cells of COCs (Cumulus-Oocyte Complexes) that are suitable for relative gene expression analyses after in vitro mat
Anim. Reprod.. Publicado em: 25/11/2019
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9. METALLOTHIONEIN-2A (RS1610216&RS28366003) GENE POLYMORPHISMS AND THE RISK OF STOMACH ADENOCARCINOMA
RESUMO CONTEXTO: O câncer gástrico é a quarta causa mais comum de câncer em todo o mundo. Também em contraste com os enormes avanços na cura, a chance de viver é muito baixa, mesmo em casos de cirurgia. Ter uma predisposição genética desempenha um papel importante no desenvolvimento do câncer. A associação entre polimorfismos do gene metalotio
Arq. Gastroenterol.. Publicado em: 07/11/2019
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10. Development of an Extraction Protocol for Fetal Sexing from the Fourth Week of Gestation by Real Time PCR
Abstract Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and clinical applications. This research developed a technology which allowed the extraction of fetal DNA by a non-invasive method that offers no risk to the mother or fetus. A total of 132 pregnant women participated in this inquiry. The DNA extraction was
Braz. arch. biol. technol.. Publicado em: 24/10/2019
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11. Molecular Diagnostics of Dengue by Reverse Transcription-Loop Mediated Isothermal Amplification (RT-LAMP) in Disposable Polyester-Toner Microdevices
Dengue is one of the most prevalent infectious tropical diseases in the world, with high incidence in over 100 countries. Rapid and reliable diagnosis of dengue is of great importance for public health. To simplify molecular diagnostics, isothermal amplification techniques have recently emerged as an alternative to conventional methods of deoxyribonucleic ac
J. Braz. Chem. Soc.. Publicado em: 16/09/2019
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12. INVESTIGATION OF ENTEROCOCCUS FAECALIS POPULATION IN PATIENTS WITH POLYP AND COLORECTAL CANCER IN COMPARISON OF HEALTHY INDIVIDUALS
RESUMO CONTEXTO: O câncer colorretal é um dos cânceres mais comumente diagnosticados em todo o mundo. Um dos fatores envolvidos no desenvolvimento do câncer colorretal é a mudança na flora normal do intestino. OBJETIVO: O número médio de cópias de Enterococcus faecalis em pessoas com pólipos e pessoas com câncer colorretal foram avaliados em c
Arq. Gastroenterol.. Publicado em: 26/08/2019