Dna Amplification Fingerprinting
Mostrando 1-12 de 86 artigos, teses e dissertações.
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1. DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae)
ABSTRACT DNA fingerprinting based on SSR amplification profiles was applied to native species of Piper from the Atlantic Forest to compare the utility of this type of molecular marker with the morphological characters traditionally applied in Piper taxonomy and identification. Fifty-one SSR markers developed for four species of Piper native to Asia and Mesoa
Acta Bot. Bras.. Publicado em: 19/03/2018
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2. Development and validation of new SSR markers from expressed regions in the garlic genome
Only a limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) despite the fact that SSR markers have become one of the most preferred DNA marker systems. To develop new SSR markers for the garlic genome, garlic expressed sequence tags (ESTs) at the publicly available GarlicEST database were screened f
Sci. agric. (Piracicaba, Braz.). Publicado em: 2015-02
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3. Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal
Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root devel
Braz. J. Microbiol.. Publicado em: 16/08/2013
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4. Diversidade GenÃtica Populacional nas espÃcies pioneiras Cyperus ligularis L. C. oduratus L. (Cyperaceae) e Miconia prasina (Sw.) dc. (Melastomataceae) ocorrentes em remanescentes da Floresta AtlÃntica de Pernambuco detectada pelos marcadores moleculares DAF e ISSR
The Atlantic Forests have suffered continuous reductions resulting in urgent need of new strategies for its preservation and for the understanding of the fragmentation consequences, especially aiming its preservation. Regarding this scenario, the plant families Cyperaceae and Melastomataceae play an important role, due to its function as pioneers and forest
Publicado em: 2009
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5. Relacionamento genÃtico de espÃcies do gÃnero Philodendron (Araceae, Monocotyledoneae) atravÃs da tÃcnica de DAF (DNA Amplification Fingerprinting)
O gÃnero Philodendron (Araceae) apresenta destacada importÃncia nÃo apenas devido a seu contingente populacional, mas tambÃm pela ampla utilizaÃÃo ornamental, devido à beleza e diversidade de formas e cores de suas folhagens. Conta com aproximadamente 600 espÃcies jà registradas, distribuindo-se endemicamente nas AmÃricas e apresentando grande dive
Publicado em: 2008
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6. RAPD fingerprinting of the ornamental fish Badis badis (Hamilton 1822) and Dario dario (Kullander and Britz 2002) (Perciformes, Badidae) from West Bengal, India
We used random amplification of polymorphic DNA (RAPD) to generate species-specific diagnostic fragment patterns for the molecular identification of the ornamental aquarium fish species Badis badis and Dario dario. Seven arbitrary oligodecamer primers produced a total of 116 bands of which 98.23% were polymorphic. The size of the amplified products was in th
Genetics and Molecular Biology. Publicado em: 2008
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7. Diversidade genÃtica e avaliaÃÃo de genÃtipos de feijÃo-caupi contrastantes para resistÃncia aos estresses biÃticos e abiÃticos com marcadores SSR, DAF e ISSR
O estudo da diversidade genÃtica entre acessos de bancos de germoplasma fornece importantes subsÃdios aos programas de melhoramento vegetal. Com a finalidade de caracterizar a diversidade genÃtica inter e intra-especÃfica do gÃnero Vigna, as tÃcnicas de DAF (DNA Amplification Fingerprinting), ISSR (Inter Simple Sequence Repeat) e SSR (Simple Sequence R
Publicado em: 2008
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8. Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban triatominae
Random amplified polymorphic DNA (RAPD) technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of primer, magnesium chloride, template DNA and Taq DNA polymeras
Revista do Instituto de Medicina Tropical de São Paulo. Publicado em: 2005-10
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9. Variabilidade genÃtica em populaÃÃes de Ricinus communis (Euphorbiaceae) pela metodologia de DAF (DNA Amplification Fingerprinting)
O Brasil ocupa mundialmente a terceira posiÃÃo na produÃÃo de mamona (Ricinus communis L.). Embora existam muitos estudos moleculares voltados Ãs proteÃnas expressas nas sementes, nÃo existem atà o momento anÃlises com marcadores moleculares avaliando a diversidade genÃtica dessa espÃcie. O presente trabalho estudou a variabilidade genÃtica de 16
Publicado em: 2004
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10. Marcadores de DNA na caracterizaÃÃo de germoplasma de feijÃo massacar (Vigna Unguiculata (L.) Walp.)
O presente estudo descreve os resultados obtidos a partir da aplicaÃÃo da metodologia de DAF (DNA Amplification Fingerprinting; ImpressÃo Digital da AmplificaÃÃo do DNA) na discriminaÃÃo de alguns acessos de feijÃo macassar (Vigna unguiculata (L.) Walp.) depositados em bancos de germoplasma. O estudo avaliou um total de 30 genÃtipos, incluindo 28 ac
Publicado em: 2003
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11. CaracterizaÃÃo genÃtica de populaÃÃes de leveduras de destilarias de Ãlcool combustÃvel para otimizaÃÃo do processo de fermentaÃÃo / Genetic caracterization of yeasts populations of fuel alcohol distilleries to otimization of fermentation process
A total of 803 yeast isolates were obtained from quarterly collected samples from fermented must from four different fuel-ethanol distilleries located in Northern Brasil: 110 isolates from Miriri distillery, 90 isolates from Giasa distillery and 443 isolates from Japungu distillery, all from State of ParaÃba, and 160 isolates from JB distillery located at t
Publicado em: 2003
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12. Fingerprinting of cell lines by directed amplification of minisatellite-region DNA (DAMD)
The development of in vitro propagation of cells has been an extraordinary technical advance for several biological studies. The correct identification of the cell line used, however, is crucial, as a mistaken identity or the presence of another contaminating cell may lead to invalid and/or erroneous conclusions. We report here the application of a DNA finge
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-11