Direct Organogenesis
Mostrando 13-24 de 29 artigos, teses e dissertações.
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13. Estudos anatomicos e ultra-estruturais da organogenese in vitro de Passiflora edulis Sims f. flavicarpa Deg / Anatomy and structural studies of in vitro organogenesis of Passiflora edulis Sims f. flavicarpa Deg
Meristemoids are responsible for the in vitro organogenesis expression. They may be formed from the explant (direct organogenesis) or from callus (indirect organogenesis). Once in vitro organogenesis is a prerequisite for developing micropropagation strategies and genetic transformation in plants, the ultrastructural analysis of the cells involved in such re
Publicado em: 2005
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14. IN VITRO SEEDS GERMINATION AND MORPHOGENESIS OF BOTTLE GOURD (Lagenaria siceraria (Mol.) Standl.) AND SQUASH (Cucurbita pepo L.) / GERMINAÇÃO IN VITRO DE SEMENTES E MORFOGÊNESE DE PORONGO (Lagenaria siceraria (Mol.) Standl.) E MOGANGO (Cucurbita pepo L.)
O estabelecimento e a germinação in vitro suprem explantes em grande quantidade para a organização de experimentos em morfogênese, a qual apresenta muitas aplicações, tais como a indução de flores in vitro, a obtenção de plantas haplóides e duplo-haplóides, a propagação clonal em massa, a embriogênese somática, entre tantas outras. O objetiv
Publicado em: 2005
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15. Caracterização anatômica da organogênese in vitro e transformação genética via Agrobacterium tumefaciens em Citrus sp. / Anatomical analysis of in vitro organogenesis and Agrobacterium tumefaciens-mediated transformation in Citrus sp.
Genetic transformation has been more frequently associated with conventional genetic breeding programs of different species. It allows for the introduction of exogenous gene(s) into the plant genome, with the possibility of altering specific characteristics. Thus, it can be an important tool for Citrus conventional breeding programs, which present several li
Publicado em: 2002
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16. A role for Timeless in epithelial morphogenesis during kidney development
Central to the process of epithelial organogenesis is branching morphogenesis into tubules and ducts. In the kidney, this can be modeled by a very simple system consisting of isolated ureteric bud (UB) cells, which undergo branching morphogenesis in response to soluble factors present in the conditioned medium of a metanephric mesenchyme cell line. By employ
The National Academy of Sciences.
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17. Regulation of human SRY subcellular distribution by its acetylation/deacetylation
SRY, a Y chromosome-encoded DNA-binding protein, is required for testis organogenesis in mammals. Expression of the SRY gene in the genital ridge is followed by diverse early cell events leading to Sertoli cell determination/differentiation and subsequent sex cord formation. Little is known about SRY regulation and its mode of action during testis developmen
Nature Publishing Group.
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18. FGF4, a direct target of LEF1 and Wnt signaling, can rescue the arrest of tooth organogenesis in Lef1−/− mice
Lymphoid enhancer factor (LEF1), a nuclear mediator of Wnt signaling, is required for the formation of organs that depend on inductive interactions between epithelial and mesenchymal tissues. In previous tissue recombination experiments with normal and Lef1−/− tooth germs, we found that the effect of LEF1 expression in the epithelium is tissue nonautonom
Cold Spring Harbor Laboratory Press.
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19. Hair-specific expression of chloramphenicol acetyltransferase in transgenic mice under the control of an ultra-high-sulfur keratin promoter.
We have generated a transgenic mouse line by microinjection of a chimeric DNA fragment (KER-CAT) containing a hair-specific, murine ultra-high-sulfur keratin promoter (KER) fused to the coding region of the bacterial chloramphenicol acetyltransferase (CAT) gene. A 671-base pair (bp) stretch of the 5' promoter region was used to direct the expression of the C
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20. The Vestigial and Scalloped proteins act together to directly regulate wing-specific gene expression in Drosophila
A small number of major regulatory (selector) genes have been identified in animals that control the development of particular organs or complex structures. In Drosophila, the vestigial gene is required for wing formation and is able to induce wing-like outgrowths on other structures. However, the molecular function of the nuclear Vestigial protein, which be
Cold Spring Harbor Laboratory Press.
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21. Nodulation Phenotypes of Gibberellin and Brassinosteroid Mutants of Pea1
The initiation and development of legume nodules induced by compatible Rhizobium species requires a complex signal exchange involving both plant and bacterial compounds. Phytohormones have been implicated in this process, although in many cases direct evidence is lacking. Here, we characterize the root and nodulation phenotypes of various mutant lines of pea
American Society of Plant Biologists.
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22. Wnt/β-Catenin/Tcf Signaling Induces the Transcription of Axin2, a Negative Regulator of the Signaling Pathway
Axin2/Conductin/Axil and its ortholog Axin are negative regulators of the Wnt signaling pathway, which promote the phosphorylation and degradation of β-catenin. While Axin is expressed ubiquitously, Axin2 mRNA was seen in a restricted pattern during mouse embryogenesis and organogenesis. Because many sites of Axin2 expression overlapped with those of severa
American Society for Microbiology.
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23. Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo
One of the first steps in zebrafish heart and gut organogenesis is the migration of bilateral primordia to the midline to form cardiac and gut tubes. The mechanisms that regulate this process are poorly understood. Here we show that the proteoglycan syndecan 2 (Sdc2) expressed in the extra-embryonic yolk syncytial layer (YSL) acts locally at the YSL-embr
Company of Biologists.
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24. Postnatal Lethality in Mice Lacking the Sax2 Homeobox Gene Homologous to Drosophila S59/slouch: Evidence for Positive and Negative Autoregulation
Homeobox gene transcription factors direct multiple functions during development. They are involved in early patterning of the embryo as well as cell specification, cell differentiation, and organogenesis. Here we describe a previously uncharacterized murine homeobox gene, Sax2, that shows high similarity to the Drosophila S59/slouch and murine Sax1 genes. W
American Society for Microbiology.