Deoxyribonucleases
Mostrando 1-12 de 34 artigos, teses e dissertações.
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1. Caracterização da DNase da peçonha da serpente Bothrops alternatus : comparação com a DNase acida de mamiferos envolvida em apoptose / Characterization of a Dnase from Bothrops alternatus snake venom : comparision with mamalian acid Dnases involved in apoptosis
Bothrops snake venoms are cytotoxic to a variety of cells (endothelial, smooth muscle, renal and inflammatory cells), and may cause cell death by apoptosis. The venom components implicated in apoptosis include metalloproteinases and L-amino acid oxidase. In contrast, although acidic deoxyribonucleases (DNase II) have been implicated in DNA fragmentation duri
Publicado em: 2008
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2. Site-Specific Deoxyribonucleases in Bacillus subtilis and Other Bacillus Strains
We systematically studied site-specific deoxyribonucleases in Bacillus strains and detected deoxyribonuclease activities in 20 of 62 strains tested.
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3. Regulated breakdown of Escherichia coli deoxyribonucleic acid during intraperiplasmic growth of Bdellovibrio bacteriovorus 109J.
During growth of Bdellovibrio bacteriovorus on [2-14C]deoxythymidine-labeled Escherichia coli, approximately 30% of the radioactivity was released to the culture fluid as nucleoside monophosphates and free bases; the remainder was incorporated by the bdellovibrio. By 60 min after bdellovibrio attack, when only 10% of the E. coli deoxyribonucleic acid (DNA) h
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4. Regulation of virus-induced deoxyribonucleases.
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5. Bacteriophage T3- and T7-directed deoxyribonucleases.
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6. Extracellular Deoxyribonucleases in Members of the Family Enterobacteriaceae
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7. Prophage induction in a permeabilized cell system: induction by deoxyribonucleases and the role of recBC-deoxyribonuclease.
Permeabilized cells able to induce prophage were obtained by plasmolysis and preincubation of the cells in a reaction mixture which allows protein synthesis. These cells became permeable to low-molecular-weight proteins and oligonucleotides. We found that deoxyribonucleases (pancreatic deoxyribonuclease and micrococcal nuclease) triggered prophage (phi 80) i
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8. Purification and properties of two deoxyribonucleases of Pseudomonas aeruginosa.
A survey of the major deoxyribonucleases in Pseudomonas aeruginosa strain PAO was undertaken. Two activities predominated in Brij-58 lysates of this organism. These have been purified from contaminating nuclease activities, and some of their properties have been elucidated. The first was a nuclease that degraded heat-denatured deoxyribonucleic acid (DNA) to
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9. Mutants of Diplococcus pneumoniae that Lack Deoxyribonucleases and Other Activities Possibly Pertinent to Genetic Transformation
Mutants of Diplococcus pneumoniae that lacked the two major deoxyribonucleases of the cell—one an endonuclease, the other an exonuclease preferentially active on native deoxyribonucleic acid (DNA)—were obtained. The development of a method for detecting mutant colonies, based on the binding of methyl green to DNA, facilitated isolation of the mutants. Ne
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10. Possible Relationship Between Yeast Deoxyribonucleases and Deoxyribonucleic Acid Yield
The deoxyribonucleic acid (DNA) yield and deoxyribonuclease (DNase) activity of several yeasts were correlated. Debaryomyces castellii and Debaryomyces franciscae were found to contain active DNases which carry out DNA hydrolysis, whereas the amounts of DNA as determined by extraction with Sarkosyl buffer (pH 7.8) were found to be small. On the other hand, C
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11. Characterization of Deoxyribonucleases Induced by Poxviruses 1
Increases in deoxyribonuclease activity assayed at alkaline pH can be observed in poxvirus-infected cells when native or denatured deoxyribonucleic acid (DNA) is used as substrate. The deoxyribonuclease assayable with native DNA as substrate, induced in HeLa cells by cowpoxvirus or vaccinia virus WR, can be separated from the corresponding enzyme present in
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12. A new assay of deoxyriboucleases using as a substrate radioactively labelled DNA bound either directly or through anti-DNA antibodies to plastic depression plates.
A simple and accurate method for the detection of Deoxyribonucleases is described. The DNA substrate (3H-labeled) is bound to DNA-binding sites in the "wells" of plastic depression plates either directly of via anti-DNA antibodies. Following incubation with the appropriate enzyme, the radioactivity released from the wells or left bound to the wells is determ