Cytological Techniques
Mostrando 13-24 de 59 artigos, teses e dissertações.
-
13. Estudo citogenetico comparativo de Edalorhina perezi e Physalaemus petersi (Amphibia, Anura, Leptodactylidae)
Sixty one specimens of Physalaemus petersi and seven of Edalorhina perezi (Leptodactylidae, Anura) from Acre State, Brazil, were studied, to contribute to phylogenetic relationship studies between these two neotropical genera. Chromosome preparations were obtained from cellular suspensions of testis and intestinal epithelium. These cytological preparations w
Publicado em: 1996
-
14. Estudos sobre a biologia reprodutiva de especies de melastomataceae de cerrado em Itirapina, SP
This study was set up to investigate some aspects of the reproductive biology of 11 species of Melastomataceae growing in cerrado vegetation, in Itirapina, SP, Brazil. These were: Leandra lacunosa Cogn., Miconia albicans (Sw.) Triana, M. fallax DC., M. langsdorffii Cogn., M. ligustroides (DC.) Naud., M. minutiflora (Bonpl.) DC., M. pepericarpa Mart. ex DC.,
Publicado em: 1994
-
15. A comparison of four cytological sampling techniques in a genitourinary medicine clinic.
Four cytological sampling techniques (the Ayre's spatula alone, the Ayelesbury spatula, the Ayre's spatula in conjunction with a Cytobrush (Medscand), and the Cervex (Steriseal] were compared when used in a genitourinary medicine clinic. Over a period of two years 6991 smears were taken. No difference was found between the methods with regard to detection of
-
16. New observations on the onset and duration of the meiotic prophase in the female golden hamster (Mesocricetus auratus).
The onset and evolution of the meiotic prophase were assessed by histological and cytological techniques in fetal and neonatal ovaries obtained from day 13 postcoitum to day 11 postpartum in the golden hamster (Mesocricetus auratus). The histological technique based on the Feulgen stain and the cytological technique based on the Giemsa stain of isolated nucl
-
17. Wash off liver cytology: a complementary diagnostic tool to liver biopsy.
Parenchymal and non-parenchymal cells were harvested by washing the liver tissue core and needle after percutaneous biopsy. The cytological material obtained was suitable for morphological analysis, including showing the presence of surface and cytoplasmic antigens using labelled antibody techniques. This technique provides a combined cytological and histolo
-
18. Miniature Tissue Culture Technique with a Modified Plastic Microplate
Plastic microplates were modified to accommodate four glass microscope slides as surfaces for cell cultivation. Tissue cultures grown on these slides can be utilized for fluorescent-antibody and other cytological staining techniques.
-
19. Cytological sampling at fibreoptic bronchoscopy: comparison of catheter aspirates and brush biopsies.
A prospective study was undertaken to compare bronchial brushings with dry catheter aspiration for the cytological diagnosis of lung cancer at fibreoptic bronchoscopy. Duplicate samples taken by aspirate and brush were obtained at 103 consecutive routine bronchoscopies. Aspirate and brush samples were reported on separately by two cytologists. At the end of
-
20. New method for preserving cytology specimens.
A method of freezing cytological material for long term storage, based on a modification of that used in tissue typing for the storage of lymphocytes, was developed. The method entails the centrifugation of the specimen, adding 1 ml AB or fetal calf serum and 1 ml 20% dimethyl sulphoxide aliquoted into 2 ml tubes, and storage at -70 degrees C. Cytological de
-
21. Immunocytochemical staining with monoclonal antibodies in cytologically "negative" serous effusions from patients with malignant disease.
In order to assess the practical value of immunocytochemical techniques in routine cytological diagnosis, a panel of three monoclonal antibodies (anti-CEA, Ca 1 and HMFG-2) were used to search for malignant cells in 53 samples of pleural or peritoneal fluid from 41 selected patients. All these patients suffered from malignant disease, but neoplastic cells ha
-
22. Meiosis in Drosophila: seeing is believing.
Recently many exciting advances have been achieved in our understanding of Drosophila meiosis due to combined cytological and genetic approaches. New techniques have permitted the characterization of chromosome position and spindle formation in female meiosis I. The proteins encoded by the nod and ncd genes, two genes known to be needed for the proper partit
-
23. Ductal approaches to assessment and management of women at high risk for developing breast cancer
The ductal approach to breast cancer, encompassing nipple aspiration, ductal lavage and duct endoscopy, allows assessment of breast ductal epithelial cells and their local microenvironment in a graded process of increasing invasiveness. Samples of ductal epithelial cells sufficient for cytological diagnosis may be safely collected, titres of individual prote
BioMed Central.
-
24. HPV testing in patients with low grade cervical cytological abnormalities: a follow up study.
AIM: To assess the diagnostic performance of human papillomavirus (HPV) analysis in predicting cervical intraepithelial neoplasia (CIN) grades 2 and 3 in patients with persistent low grade cervical cytological abnormalities. METHODS: Cervical smears from 167 women referred for colposcopy with persistent borderline, wart virus or mildly dyskaryotic changes on