Cumulus Cells
Mostrando 1-12 de 47 artigos, teses e dissertações.
-
1. ADAMTS proteoglycanases downregulation with impaired oocyte quality in PCOS
ABSTRACT Objective: A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) and ADAMTS-5 normal expression levels are essential for ovulation and subsequent fertilization. The objective of the present study was to assess expression pattern of these genes in cumulus cells (CCs) taken from patients with polycystic ovary syndrome (PCOS) and
Arch. Endocrinol. Metab.. Publicado em: 2021-02
-
2. Intrafollicular barriers and cellular interactions during ovarian follicle development
Abstract Follicles are composed of different interdependent cell types including oocytes, cumulus, granulosa, and theca cells. Follicular cells and oocytes exchange signaling molecules from the beginning of the development of the primordial follicles until the moment of ovulation. The follicular structure transforms during folliculogenesis; barriers form bet
Anim. Reprod.. Publicado em: 28/11/2019
-
3. Validation of reference genes for gene expression studies in bovine oocytes and cumulus cells derived from in vitro maturation
Abstract Quantitative real-time PCR (qPCR) is a valuable tool for gene expression studies and it is necessary to choose an ideal endogenous reference gene for data normalization. This work studied a set of reference genes in oocytes and cumulus cells of COCs (Cumulus-Oocyte Complexes) that are suitable for relative gene expression analyses after in vitro mat
Anim. Reprod.. Publicado em: 25/11/2019
-
4. Effect of Fibroblast Growth Factor 2 (FGF2) and Insulin Transferrin Selenium (ITS) on In Vitro Maturation, Fertilization and Embryo Development in Sheep
The present study evaluated the effect of fibroblast growth factor 2 (FGF2) and insulin-transferrin-selenium (ITS) to the in vitro maturation and embryo culture media on ovine oocyte maturation, cleavage and embryo development. Oocytes having more than five layers of unexpanded cumulus cells and granular homogenous ooplasm were cultured into 50 μL droplets
Braz. arch. biol. technol.. Publicado em: 2015-08
-
5. Análise do mRNA da proteína BMP-15 e IGF-2 em oócitos e células do cumulus de ovelhas do semiárido pernambucano. / Analysis of mRNA expression of BMP-15 and IGF-2 proteins in oocytes and cumulus cells of sheep in semi-arid state of Pernambuco.
Two experiments were conducted to analyze the expression of genes related to follicular development and subsequent reproductive efficiency in oocytes and cumulus cells in two distinct phases of folliculogenesis. Ovaries of native breeds of sheep slaughtered in the abattoir in Caruaru/PE were collected and transported to the Laboratory of Applied Molecular An
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/02/2012
-
6. Avaliação da Proporção Sexual de Embriões Desenvolvidos In Vitro e de Progênie a Campo de Touros Jovens. / Evaluation of the Sex Ratio of Embryos Developed In Vitro and in Field Progeny of Young Sires.
Some of the main problems that affect the in vitro production of embryos is the variation among bulls in relation to fertility and the greater birth of male embryos. Many factors can change the ratio 1:1 between the genders, both in the in vitro production of embryos and in the method of artificial insemination. To try to change this sex ratio in vitro, we s
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/08/2011
-
7. Avaliação da expressão gênica e da maturação nuclear in vitro em complexos cumuli-oócitos bovinos
Dentre os principais desafios que persistem no campo da biologia da reprodução está a compreensão da natureza dos processos celulares e moleculares que determinam a qualidade dos oócitos. Um dos fenômenos a serem melhor compreendidos é a aquisição da competência do oócito, e qual o papel desempenhado pelo ambiente folicular que circunda o gameta n
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 2011
-
8. Expressão gênica das células do cumulus oophorus de bovinos após vitrificação = Gene expression of bovine cumulus oophorus cells after vitrification / Gene expression of bovine cumulus oophorus cells after vitrification
Um dos desafios da criobiologia continua sendo o desenvolvimento de um método que proporcione a manutenção da viabilidade após a criopreservação de oócitos imaturos na espécie bovina. A vitrificação tem sido a metodologia que proporciona resultados de sobrevivência após a criopreservação mais promissores para complexos cumulus-oócito (CCOs) im
Publicado em: 2010
-
9. Efeito de Diferentes Concentrações de Oxigênio Sobre o Cultivo In Vitro de Folículos Pré-Antrais Caprinos. / Effect of Different Concentrations of Oxygen About In Vitro Cultivation of primordial follicles Goats.
The aim of the present study was to evaluate the effect of two different oxygen (O2) concentrations on the survival and development of goat preantral follicles cultured in vitro. Preantral ovarian follicles (150 μm) were isolated from ovarian cortex fragments and individually cultured for 30 days under two different O2 concentrations (5% and 20%). Folli
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 07/12/2009
-
10. Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development
Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the cu
Brazilian Journal of Medical and Biological Research. Publicado em: 2009-06
-
11. RT-PCR QUANTITATIVO EM TEMPO REAL PARA ANÁLISE DO RECEPTOR DE EGF EM COMPLEXOS CUMULUS-OÓCITO COLHIDOS POR LAPAROSCOPIA EM CABRAS CANINDÉ SUBMETIDAS À ESTIMULAÇÃO HORMONAL OVARIANA
Ovarian stimulation with exogenous Follicle Stimulating Hormone (FSH) has been used to increase the number of viable oocytes for laparoscopic oocyte recovery (LOR) in goats. The aim of this study was to evaluate the effect of two FSH protocols for ovarian stimulation in goats on the expression pattern of EGF receptor (EGFR) in cumulus-oocyte complexes (COCs)
Publicado em: 2009
-
12. Expressão de genes candidatos para a aquisição da competência ovocitária em bovinos
The success of the various assisted reproductive techniques depends on oocyte competence. However, the factors involved on its acquisition and the molecular attributes of competent oocyte are generally unknown. In an attempt to assess genes related with oocyte competence we quantify transcripts for candidate genes in oocytes (H1Foo, H2A, H3A, GHR, GDF9, BMP1
Publicado em: 2009