Culture Media Isolation Purification
Mostrando 1-8 de 8 artigos, teses e dissertações.
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1. Produção, isolamento e identificação de metabólito com atividade citotóxica de cultura de Streptomyces carpaticus / Production, isolation and identification of citotoxic activity metabolite of streptomyces carpaticus culture
This study addresses the production, isolation and identification of the substance present in culture broth of Streptomyces carpaticus which showed high biological activity against human tumor cell lines HL-60 (promyelocytic leukemia), MDA-MB 435 (breast carcinoma ), HCT-8 (colon carcinoma), SF-295 (glioblastoma). Cultures of Streptomyces carpaticus in cultu
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 21/09/2011
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2. InfluÃncia do meio de cultura na produÃÃo de metabÃlicos bioativos pelo endÃfito Streptomycessp. EBR49-A UFPEDA
Endophytic Streptomyces strains of medicinal plants has been contributing in a meaningful whit promising source for discovery of new bioactive metabolites. This study has as objective to select the best culture media and time of production of secondary metabolites then your isolation. Five different fermentation media were used in through the disk diffusion
Publicado em: 2009
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3. Onychomycosis caused by filamentous fungi non-dermatophytes / Onicomicoses causadas por fungos filamentosos não dermatófitos
INTRODUCTION: Onychomycosis, a nail fungus infection is the most frequent nail disease, constituting about half of all nail disorder. It can be caused by dermatophytes, yeasts and non- dermatophytes filamentous fungi. OBJECTIVE: Characterize the onychomycosis caused by filamentous fungi non- dermatophytes. (1) Verify after a clinical suspicion of onychomycos
Publicado em: 2008
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4. Growth in serum-free medium improves isolation of Chlamydia pneumoniae.
Infectivity titers were determined for eight Chlamydia pneumoniae strains simultaneously grown in serum-free and serum-supplemented cell culture media. Use of serum-free medium resulted in a 10- to 50-fold increase in the susceptibility of HL cells to chlamydial infection. Comparative primary isolation of a wild-type strain also produced higher inclusion cou
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5. Laboratory and field evaluation of selective media for isolation of group B streptococci.
Problems encountered with currently recommended selective media for group B streptococci (GBS) (selective broth medium and CNA agar) prompted a searach for alternative culture methods in ongoing epidemiological studies. Previously recommended inhibitory agents were tested in vitro. Gentamicin, alone or in combination with nalidixic acid, proved inhibitory fo
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6. Comparative production and rapid purification of Candida acid proteinase from protein-supplemented cultures.
Six Candida spp. that were previously characterized for cutaneous pathogenicity were assessed for Candida acid proteinase (CAP) production in albumin-supplemented, nitrogen-restricted media. C. albicans CAP production was compared in media supplemented with albumin, casein, collagen, hemoglobin, or keratin and in TC medium 199. C. albicans, C. stellatoidea,
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7. Isolation and characteristics of collagenolytic enzyme produced by Candida albicans.
In media containing collagen as the nitrogen source, the pathogenic yeast Candida albicans secreted a collagenolytic enzyme. Purification of the enzyme from a culture filtrate was achieved by DEAE-Sephacel chromatography at pH 6.7. The molecular weight was found to be 46,000 by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the isoelectri
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8. A food-grade process for isolation and partial purification of bacteriocins of lactic acid bacteria that uses diatomite calcium silicate.
Bacteriocins, including nisin, pediocin PO2, brevicin 286, and piscicolin 126, were extracted from fermentation media by adsorption onto Micro-Cel (a food-grade diatomite calcium silicate anticaking agent) and subsequent desorption. The optimal conditions for desorption of piscicolin 126 were determined and applied to other bacteriocins, and the relative pur