Culture Broth
Mostrando 13-24 de 1076 artigos, teses e dissertações.
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13. Rapid Detection of ACTG- and AK-Toxins in Alternaria alternata by LC-ESI-MS/MS Analysis and Antifungal Properties of Citrus Compounds
A methanol-soluble fraction of the dichloromethane extract from the culture broth of A. alternata AT4303 strain afforded the following five known mycotoxins: alternariol, alternariol monomethyl ether, altenusin, altenuene and altertoxin I. A hexane-soluble fraction of the dichloromethane extract yielded the steroids ergosterol and ergosterol peroxide, and al
J. Braz. Chem. Soc.. Publicado em: 2016-08
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14. Endophytic fungus strain 28 isolated from Houttuynia cordata possesses wide-spectrum antifungal activity
Abstract The aim of this paper is to identify and investigate an endophytic fungus (strain 28) that was isolated from Houttuynia cordata Thunb, a famous and widely-used Traditional Chinese Medicine. Based on morphological methods and a phylogenetic analysis of ITS sequences, this strain was identified as Chaetomium globosum. An antifungal activity bioassay d
Braz. J. Microbiol.. Publicado em: 2016-06
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15. Screening of Trichoderma isolates for their potential of biosorption of nickel and cadmium
Abstract Fourteen Trichoderma isolates were evaluated for their tolerance to two heavy metals, nickel and cadmium. Three isolates, MT-4, UBT-18, and IBT-I, showed high levels of nickel tolerance, whereas MT-4, UBT-18, and IBT-II showed better tolerance of cadmium than the other isolates. Under nickel stress, biomass production increased up to a Ni concentrat
Braz. J. Microbiol.. Publicado em: 2016-06
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16. The genotypic diversity and lipase production of some thermophilic bacilli from different genera
Abstract Thermophilic 32 isolates and 20 reference bacilli were subjected to Rep-PCR and ITS-PCR fingerprinting for determination of their genotypic diversity, before screening lipase activities. By these methods, all the isolates and references could easily be differentiated up to subspecies level from each other. In screening assay, 11 isolates and 7 refer
Braz. J. Microbiol.. Publicado em: 2015-12
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17. Molecular variations in Vibrio alginolyticus and V. harveyi in shrimp-farming systems upon stress
A study was performed to investigate the genomic variations in the shrimp farm isolates of Vibrio alginolyticus and V. harveyi when the isolates were subjected to environmental stress. Samples of shrimps, water and sediment were collected from Southern Indian coastal shrimp farms. Vibrio isolates were biochemically identified and confirmed using 16S rDNA and
Braz. J. Microbiol.. Publicado em: 27/10/2015
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18. Gas Chromatography - Mass Spectrometry Analysis and Antibacterial Activity of Bluish-Green Pigment from Pseudomonas sp. JJTBVK (KF836502)
The present study was conducted for the isolation of potential bacteria from the desert soil, their molecular identification and prediction of restriction sites of the potential isolate using the bioinformatics tools. Production of the metabolites was done by inoculating in nutrient broth of pH 8.6. Metabolite was bluish-green in color; it was extracted and
Braz. arch. biol. technol.. Publicado em: 2015-08
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19. Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units
In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae. Three different phenotypic methods were evaluated: (1) combined-disk test of meropenem plus phenylboronic acid or EDTA reading after 24 h and 48 h; (2) selective/chromogenic read after 24 h and after 48 h; and (3) overnight sel
Braz J Infect Dis. Publicado em: 2015-08
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20. A new improved protocol for in vitro intratubular dentinal bacterial contamination for antimicrobial endodontic tests: standardization and validation by confocal laser scanning microscopy
Objectives To compare three methods of intratubular contamination that simulate endodontic infections using confocal laser scanning microscopy (CLSM). Material and Methods Two pre-existing models of dentinal contamination were used to induce intratubular infection (groups A and B). These methods were modified in an attempt to improve the model (group C).
J. Appl. Oral Sci.. Publicado em: 21/07/2015
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21. Sub-MIC of antibiotics induced biofilm formation of Pseudomonas aeruginosa in the presence of chlorhexidine
Public health is facing a new challenge due to the alarming increase in bacterial resistance to most of the conventional antibacterial agents. It has been found that only minor cell damage is caused when exposed to sub-lethal levels of antimicrobial. Biofilms can play an important role in producing resistance, which is developed to reservoirs of pathogens in
Braz. J. Microbiol.. Publicado em: 2015-03
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22. RECOVERY OF CYCLODEXTRIN GLUCANOTRANSFERASE (CGTase) USING IMMOBILIZED METAL CHELATING AFFINITY CHROMATOGRAPHY
Abstract Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSkin dialysis tubing (3.5 MWCO) with an enzyme activit
Braz. J. Chem. Eng.. Publicado em: 2015-03
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23. Different methods to quantify Listeria monocytogenesbiofilms cells showed different profile in their viability
Listeria monocytogenes is a foodborne pathogen able to adhere and to form biofilms in several materials commonly present in food processing plants. The aim of this study was to evaluate the resistance of Listeria monocytogenes attached to abiotic surface, after treatment with sanitizers, by culture method, microscopy and Quantitative Real Time Polymerase Cha
Braz. J. Microbiol.. Publicado em: 2015-03
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24. Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B1
This study aimed to compare Lactobacillus rhamnosus growth in MRS (de Man, Rogosa and Sharpe) broth and a culture medium containing milk whey (MMW) and to evaluate aflatoxin B1 (AFB1) adsorption capacity by bacterial cells produced in both culture media. L. rhamnosus cells were cultivated in MRS broth and MMW (37 °C, 24 hours), and bacterial cell concentrat
Food Sci. Technol. Publicado em: 2014-09