Constitutive Expression
Mostrando 1-12 de 2433 artigos, teses e dissertações.
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1. Exercícios Ambulatórios para uma Reflexão acerca das Psicologias Sociais e da Cidade*
Resumo Penso a relação entre diferentes psicologias sociais e as cidades. Para tanto, caracterizo tais psicologias como manifestantes que foram às ruas em 2013 nas chamadas “jornadas de junho”. Faço, a partir desse experimento ficcional, uma reflexão acerca do compromisso ético e dos desafios práticos e epistêmicos que as psicologias sociais enfr
Psicol. cienc. prof.. Publicado em: 20/12/2019
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2. DIALÉCTICA NEGATIVA EN MERLEAU-PONTY*
ABSTRACT We intend to show that the philosophy of Merleau-Ponty embodies the fundamental experience of the dialectic: To bring concept to the meaning of things, the world, from the others, through an experience (Er-fahrung) expression that does not convert the sense of these in a simple result of its reflection. For him this dialectic is resolved as negative
Kriterion. Publicado em: 13/05/2019
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3. Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
The Bacille Calmette-Guérin (BCG) vaccine comprises a family of genetically different strains derived by the loss of genomic regions (RDs) and other mutations. In BCG Moreau, loss of RD16 inactivates rv3405c * , encoding a transcriptional repressor that negatively regulates the expression of Rv3406, an alkyl sulfatase. To evaluate the impact of this loss o
Mem. Inst. Oswaldo Cruz. Publicado em: 11/10/2018
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4. Impact Damage Analysis with Stress Continuity Constraints Fulfilment at Damaged-Undamaged Regions and at Layer Interfaces
Abstract Low-velocity impacts have a relevant importance for safety of laminated and sandwich composite structures, because they are highly susceptible to damage. In this paper, a 3D cost effective zig-zag model is developed in order to efficiently simulate such impacts. It a priori fulfills the continuity of out-of-plane stresses at layer interfaces, the co
Lat. Am. j. solids struct.. Publicado em: 2017-08
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5. Participation of the arcRACME protein in self-activation of the arc operon located in the arginine catabolism mobile element in pandemic clone USA300
ABSTRACT Staphylococcus aureus pandemic clone USA300 has, in addition to its constitutive arginine catabolism (arc) gene cluster, an arginine catabolism mobile element (ACME) carrying another such cluster, which gives this clone advantages in colonisation and infection. Gene arcR, which encodes an oxygen-sensitive transcriptional regulator, is inside ACME an
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-07
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6. Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean
Abstract Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the
Genet. Mol. Biol.. Publicado em: 27/03/2017
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7. Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-02
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8. Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil
Abstract Introduction There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by suscep
Braz J Infect Dis. Publicado em: 2016-06
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9. The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis
Coagulase-negative staphylococci, particularly Staphylococcus epidermidis, can be regarded as potential reservoirs of resistance genes for pathogenic strains, e.g., Staphylococcus aureus. The aim of this study was to assess the prevalence of different resistance phenotypes to macrolide, lincosamide, and streptogramins B (MLSB) antibiotics among erythromycin-
Mem. Inst. Oswaldo Cruz. Publicado em: 2016-03
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10. Use of green fluorescent protein to monitor
Lactobacillus plantarum in the gastrointestinal tract of goatsThe experiment aimed to specifically monitor the passage of lactobacilli
in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter fromL. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated intoBraz. J. Microbiol.. Publicado em: 2015-09
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11. Copper induction and differential expression of laccase in Aspergillus flavus
Aspergillus flavus was isolated from soil and exhibited laccase activity under both constitutive and copper induced conditions. Spiking the medium with 1 mM copper sulfate resulted in an increase in the activity which reached 51.84 U/mL, a distinctive protein band was detected at 60 kDa. The extracellular enzyme was purified 81 fold using gel filtration chro
Braz. J. Microbiol.. Publicado em: 2015-03
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12. Molecular cloning and mRNA expression of the peptidoglycan recognition protein gene HcPGRP1 and its isoform HcPGRP1a from the freshwater mussel Hyriopsis cumingi
Peptidoglycan recognition proteins (PGRPs) are innate immune molecules that have been structurally conserved throughout evolution in invertebrates and vertebrates. In this study, peptidoglycan recognition protein HcPGRP1 and its isoform HcPGRP1a were identified in the freshwater mussel Hyriopsis cumingii. The full-length cDNAs of HcPGRP1 (973 bp) and HcPGRP1
Genet. Mol. Biol.. Publicado em: 2014-09