Competitive Elisa
Mostrando 13-24 de 139 artigos, teses e dissertações.
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13. Purificação da glicoproteina G (GPV) recombinante do virus da raiva produzida por celulas de Drosophila melanogaster S2 atraves de cromatografia de afinidade por ions metalicos imobilizados / Purification of the recombinant rabies virus G glycoprotein (GPV) produced by Drosophila melanogaster S2 cells using immobilized metal ion affinity chromatography
Rabies or hydrophobia is a viral infection that affects the central nervous system, occuring in animals and humans. The main proteins found in rabies virus that activates the immunological system are the N nucleoprotein (NPV) and the G glycoprotein, a transmembrane protein that forms the spikes of the virus and induces virus-neutralizing antibodies that prot
Publicado em: 2007
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14. Identification of two potential receptor-binding sites for hGM-CSF
Two receptor-binding sites for hGM-CSF are described. Competitive binding ELISA using four monoclonal antibodies (MAbs) showed different epitope recognitions. The antibody combining sites were mapped using sets of overlapping peptides and hexapeptide libraries prepared by the SPOT synthesis technique. We identified the conformationally dependent epitopes A18
Brazilian Journal of Chemical Engineering. Publicado em: 2003-03
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15. Assessment of a Treponemal Competitive Enzyme Immunoassay for Syphilis Antibody Screening in 23,531 Serum Samples from a Low Prevalence Population. / Avaliação do uso de teste treponêmico imunoenzimático competitivo na triagem sorológica da sífilis em 23.531 soros de uma população de baixa prevalência
Foram testadas, com o teste não treponêmico VDRL e com o teste treponêmico imunoenzimático de competição, 23.531 amostras de soros, coletados em todas as regiões do Brasil, com o objetivo de verificar o comportamento do teste imunoenzimático treponêmico na triagem de amostras. A prevalência obtida foi de 0,63% com o VDRL e de 0,84% para o teste imu
Publicado em: 1999
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16. Competitive enzyme immunoassays for the rapid detection of antibodies to feline infectious peritonitis virus polypeptides.
Monoclonal antibodies specific for the envelope (E1), peplomer (E2), and nucleocapsid (N) polypeptides of feline infectious peritonitis virus (FIPV) were used in rapid, competitive enzyme-linked immunosorbent assays (ELISA) to study the humoral immune response of cats to FIPV infection. Results from the competitive ELISAs were correlated with those from immu
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17. A Competitive Enzyme-Linked Immunosorbent Assay for Measuring the Levels of Serum Antibody to Haemophilus influenzae Type b
A competitive ELISA method is described for the measurement of total antibodies to the capsular polysaccharide of Haemophilus influenzae type b (HibCPS) in human sera. The competitive method showed an excellent correlation to the radioantigen binding assay (RABA, or Farr assay) and improved correlation of sera with low titers with respect to the more convent
American Society for Microbiology.
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18. Antibody response to Brucella outer membrane proteins in bovine brucellosis: immunoblot analysis and competitive enzyme-linked immunosorbent assay using monoclonal antibodies.
Sera from Brucella-infected bovines were analyzed by immunoblotting by using sonicated cell extracts of B. melitensis or B. abortus and a competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies against outer membrane proteins (OMPs) with molecular masses of 10, 16.5, 19, 25 to 27, 36 to 38, and 89 kDa. Antibody responses against OMPs
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19. National serologic survey for trichinellosis in sows in Canada 1996–1997
Sera from 14 408 market sows from the Canadian domestic swine herd were tested for trichinellosis using an indirect-ELISA as a screening test and a competitive ELISA for confirmatory testing. Three sera (0.02%) gave low level reactions on the competitive ELISA. These reactions were considered nonspecific, and this designation was supported by data from previ
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20. Performance of Competitive and Indirect Enzyme-Linked Immunosorbent Assays, Gel Immunoprecipitation with Native Hapten Polysaccharide, and Standard Serological Tests in Diagnosis of Sheep Brucellosis
Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the mos
American Society for Microbiology.
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21. Detection of Glycosylated and Deglycosylated Extensin Precursors by Indirect Competitive ELISA 12
A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of the glycosylated and deglycosylated forms of the monomeric soluble extensin precursor subunits P1 and P2. A log-linear response range for each kind of precursor in the competition curve was between 0.01 and 100 nanograms per milliliter.
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22. Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay.
A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixation test results agreed in 94% (144) of the serum samples te
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23. A novel quantitative immunoassay system for p53 using antibodies selected for optimum designation of p53 status.
AIM: To develop a highly sensitive and specific enzyme linked immunosorbent assay (ELISA) system for analysis of p53 protein in cancer lysates. METHODS: The anti-p53 monoclonal antibodies DO7, 1801, BP53.12, and 421, and anti-p53 polyclonal antiserum CM1 were assessed by immunohistochemistry and western blot analysis to identify those most suitable for deter
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24. Indirect enzyme-linked immunosorbent assay for the mycotoxin zearalenone.
A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of zearalenone, an estrogenic mycotoxin. Zearalenone was converted to zearalenone-6'-carboxymethyloxime and conjugated to bovine serum albumin and poly-L-lysine for use as immunogen and solid-phase marker, respectively. Immunization of rabbits with the bovine ser