Classical Swine Fever
Mostrando 1-12 de 45 artigos, teses e dissertações.
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1. Risk of exposure of farms and subsistence nurseries to contact with wild boar in southern Mato Grosso do Sul
RESUMO: Com o avanço da distribuição do javali no ambiente rural, seus impactos não se restringem somente a sanidade suidea, embora as exigências quanto ao monitoramento e controle da espécie sejam exigências previstas pela OIE, para o reconhecimento do status de zona livre de peste suína clássica. A construção de modelos ecológicos de favorabili
Pesq. Vet. Bras.. Publicado em: 2019-02
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2. Diagnóstico da peste suína clássica nas regiões norte e nordeste do Brasil no período de 1999 a 2009.
O vírus da peste suína clássica causa doença altamente contagiosa em suínos domésticos e selvagens, caracterizada por levar a grandes perdas econômicas, tanto para a suinocultura de grande porte como também para os pequenos produtores. A doença pode se apresentar de forma aguda, crônica, tardia, assintomática ou persistente, que dependem da virul�
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 2012
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3. Rapid Detection of Classical Swine Fever Virus by a Portable Real-Time Reverse Transcriptase PCR Assay
A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR assay for classical swine fever virus (CSFV) was developed and evaluated in experimentally infected swine. The assay detected CSFV, representing different phylogenetic groupings, but did not amplify viral RNA from related pestiviruses. The assay met or exceeded the sensitivity (1 to 100 50% ti
American Society for Microbiology.
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4. Classical swine fever virus: independent induction of protective immunity by two structural glycoproteins.
To study which proteins of classical swine fever virus (CSFV) are able to confer protective immunity in swine, N-terminal autoprotease, viral core protein, and the three structural glycoproteins were expressed via vaccinia virus recombinants (VVR). CSFV proteins synthesized in cells infected with VVR showed migration characteristics on sodium dodecyl sulfate
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5. Cytopathogenicity of classical swine fever virus caused by defective interfering particles.
For three independent cytopathogenic isolates of classical swine fever virus, defective RNAs were found in infected cells in addition to full-length viral genomes. These RNAs represent the genomes of typical defective interfering (DI) particles because of strict dependence on a complementing helper virus and interference with the replication of the helper vi
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6. Classical Swine Fever Virus Leader Proteinase Npro Is Not Required for Viral Replication in Cell Culture
The sequence encoding the viral leader proteinase Npro was replaced by the murine ubiquitin gene in a full-length cDNA clone of the classical swine fever virus (CSFV) strain Alfort/187. The recombinant virus vA187-Ubi showed growth characteristics similar to those of the parent vA187-1 virus. At two occasions cells infected with vA187-Ubi exhibited a cytopat
American Society for Microbiology.
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7. RNase of classical swine fever virus: biochemical characterization and inhibition by virus-neutralizing monoclonal antibodies.
The structural glycoprotein E0 of classical swine fever virus (CSFV) possesses an intrinsic RNase activity. Here we present the first comprehensive biochemical characterization of E0, using a recombinant glycoprotein expressed in insect cells. We were able to show that the presence of neither carbohydrate moieties nor disulfide bonds is a prerequisite for RN
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8. Pathogenesis of classical swine fever: B-lymphocyte deficiency caused by hog cholera virus.
Hog cholera, also known as classical or European swine fever, is caused by hog cholera virus, a member of the genus Pestivirus. It is shown here that the end stage of lethal infection in the natural host is associated with a dramatic depletion preferentially of B lymphocytes in the circulatory system as well as in lymphoid tissues. Already at the onset of di
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9. Diagnostic Evaluation of a Real-Time Reverse Transcriptase PCR Assay for Detection of Classical Swine Fever Virus
A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase (RT) PCR for classical swine fever virus (CSFV) was evaluated for diagnostic sensitivity and specificity by using clinical samples obtained from the Dominican Republic, where the disease is enzootic. The sensitivity of this test, using nasal swab samples taken from both symptomatic and asymptomati
American Society for Microbiology.
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10. Lymphocyte Apoptosis during Classical Swine Fever: Implication of Activation-Induced Cell Death
Infection of pigs with classical swine fever virus (CSFV), a member of the Flaviviridae family, causes a severe leukopenia, particularly notable with the lymphocytes. The goal of this study was to analyze mechanisms behind this CSFV-induced lymphopenia. To this end, the kinetics of leukocyte depletion, the appearance of apoptotic cells, and virus infection o
American Society for Microbiology.
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11. Processing of pestivirus polyprotein: cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus.
The polyprotein of classical swine fever virus starts with the nonstructural protein p23, which is followed by the nucleocapsid protein p14. Proteolytic cleavage between p23 and p14 was demonstrated in a cell-free transcription-translation system. Successive truncation of the cDNA used for the transcription indicated that the proteolytic activity responsible
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12. A Recombinant Classical Swine Fever Virus Stably Expresses a Marker Gene
The gene coding for bacterial chloramphenicol acetyltransferase (CAT) was inserted in frame into the viral Npro gene of the full-length cDNA clone pA187-1 of the classical swine fever virus (CSFV) strain Alfort/187. RNA transcribed in vitro from the resulting plasmid was transfected into SK-6 porcine kidney cells. Infectious progeny virus vA187-CAT recovered
American Society for Microbiology.