Cgtase Purification
Mostrando 1-3 de 3 artigos, teses e dissertações.
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1. RECOVERY OF CYCLODEXTRIN GLUCANOTRANSFERASE (CGTase) USING IMMOBILIZED METAL CHELATING AFFINITY CHROMATOGRAPHY
Abstract Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSkin dialysis tubing (3.5 MWCO) with an enzyme activit
Braz. J. Chem. Eng.. Publicado em: 2015-03
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2. Purificação e caracterização de ciclodextrina glicosiltransferase produzida por Stenotrophomonas maltophilia / Purification and characterization of cyclodextrin glycosyltransferase produced by stenotrophomonas maltophilia isolated from brazilian soil
A ciclodextrina glicosiltransferase (EC 2.4.1.19) é a única enzima capaz de converter amido e açúcares relacionados em ciclodextrinas através de reação de ciclização. Estes compostos podem formar complexos de inclusão com moléculas hidrofóbicas, tornando-se importante para aplicação nas indústrias alimentícias, farmacêuticas, agrícolas, qu�
Publicado em: 2010
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3. Purification and Characterization of an Extremely Thermostable Cyclomaltodextrin Glucanotransferase from a Newly Isolated Hyperthermophilic Archaeon, a Thermococcus sp.
The extremely thermophilic anaerobic archaeon strain B1001 was isolated from a hot-spring environment in Japan. The cells were irregular cocci, 0.5 to 1.0 μm in diameter. The new isolate grew at temperatures between 60 and 95°C (optimum, 85°C), from pH 5.0 to 9.0 (optimum, pH 7.0), and from 1.0 to 6.0% NaCl (optimum, 2.0%). The G+C content of the genomic
American Society for Microbiology.