Cell Culture
Mostrando 1-12 de 11180 artigos, teses e dissertações.
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1. Three-dimensional cell culture for the study of nasal polyps
Abstract Objectives: Three-dimensional (3D) cell cultures have many applications such as stem cell biology research, new drug discovery, cancer, and Chronic Rhinosinusitis with Nasal Polyps (CRSwNP). This disease is characterized by a significant impact on quality of life and productivity. The diversity of factors that act in the progression of CRSwNP point
Brazilian Journal of Otorhinolaryngology. Publicado em: 2022
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2. The effects of thymoquinone on pancreatic cancer and immune cells
SUMMARY OBJECTIVES: Black cumin is widely used as a spice and as a traditional treatment. The active ingredient in black cumin seeds is thymoquinone. Thymoquinone has shown anticancer effects in some cancers. We planned to investigate its anticancer effect on pancreatic cancer cell lines. METHODS: Thymoquinone chemical component in various doses was prepar
Revista da Associação Médica Brasileira. Publicado em: 2022
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3. Expansion of cord blood stem cells in fibronectin-coated microfluidic bioreactor
ABSTRACT Background: Hematopoietic stem/progenitor cell transplantation is the main treatment option for hematological malignancies and disorders. One strategy to solve the problem of low stem cell doses used in transplantation is pre-transplant expansion. We hypothesized that using fibronectin-coated microfluidic channels would expand HSPCs and keep self-r
Hematology, Transfusion and Cell Therapy. Publicado em: 2022
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4. Evaluation of different protocols for culturing mesenchymal stem cells derived from murine bone marrow
ABSTRACT Introduction: Culturing bone marrow mesenchymal stem cells (BM-MSCs) is a key point in different fields of research, including tissue engineering and regenerative medicine and studies of the bone marrow microenvironment. However, isolating and expanding murine BM-MSCs in vitro has challenged researchers due to the low purity and yield of obtained c
Hematology, Transfusion and Cell Therapy. Publicado em: 2022
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5. Optimizing the recovery of peripheral blood mononuclear cells trapped in leukoreduction filters - A comparison study
Abstract Introduction The isolation of captured peripheral blood mononuclear cells (PBMNCs) from leukoreduction filters (LRFs) can be of great importance in terms of bringing the lost cells back into use. Objective The aim of this study was to evaluate various methods based on their potential to recover the peripheral blood cells from LRFs with a focus on
Hematology, Transfusion and Cell Therapy. Publicado em: 2022
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6. Impact of lifestyle in prostate cancer patients. What should we do?
ABSTRACT Objective: This review aimed to analyze interventions raised within primary and tertiary prevention concerning the disease's incidence, progression, and recurrence of Prostate Cancer (PCa). Priority was given to the multidisciplinary approach of PCa patients with an emphasis on modifiable risk factors. Materials and Methods: We conducted a compreh
International braz j urol. Publicado em: 2022
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7. Fibroblast morphology, growth rate and gene expression in facial melasma
Abstract Background: In addition to melanocytic hyperfunction, changes are observed in the upper dermis of melasma, and fibroblasts play a central role in collagen synthesis and pigmentation induction. Objective: To explore the morphology, growth rate, and gene expression profile of fibroblasts from the skin with melasma in comparison to fibroblasts from t
Anais Brasileiros de Dermatologia. Publicado em: 2022
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8. Generation of hematopoietic stem/progenitor cells with sickle cell mutation from induced pluripotent stem cell in serum-free system
ABSTRACT Introduction Sickle cell disease (SCD) is a monogenic disease and it is estimated that 300,000 infants are born annually with it. Most treatments available are only palliative, whereas the allogeneic hematopoietic stem cell transplantation offers the only potential cure for SCD. Objective Generation of human autologous cells, when coupled with ind
Hematol., Transfus. Cell Ther.. Publicado em: 2021-06
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9. Self-Assembled Nanoparticles of Diruthenium(II,III)-Ibuprofen Anticancer Metallodrug
The diruthenium(II,III)-ibuprofen (RuIbp) metallodrug shows anticancer activity in vitro and in vivo. The encapsulation into lipid nanoparticles enhanced the efficacy in glioblastoma, breast and prostate cancer cells. Here we report data for RuIbp hydrophobicity, solubility in alcohol/water, and behavior in 1-5% ethanol/water and ethanol/cell culture medium.
J. Braz. Chem. Soc.. Publicado em: 2020-11
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10. “Half-Sandwich”/RuII Anticancer Complexes Containing Triphenylphosphine and p-Substituted Benzoic Acids
Mononuclear and binuclear RuII/arene/triphenylphosphine complexes with p-substituted benzoic acid derivatives were prepared and characterized. These monocationic complexes of type [Ru(η6-p-cymene)(PPh3)L] (L = benzoic acid (1), p-hydroxybenzoic acid (2), p-nitrobenzoic acid (3) and terephthalic acid (4)) were characterized using various techniques, such as
J. Braz. Chem. Soc.. Publicado em: 2020-11
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11. Phylogenetic placement of Tritirachium strains from the URM culture collection originally founded by Augusto Chaves Batista (1916-1967) in Brazil, and the description of T. batistae sp. nov.
ABSTRACT Twenty-seven Tritirachium strains were present in the URM culture collection originally founded in Brazil by Augusto Chaves Batista. Fifteen freshly-prepared cultures were obtained from these original strains preserved under mineral oil. DNA was extracted for analysing phylogenetic relationships using the sequence information available from Tritirac
Acta Bot. Bras.. Publicado em: 2020-06
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12. Study of Dosage-Dependent Effects of Cytostatic Drugs Using a Fibroblast Cell Culture of the Human Nasal Mucosa
Abstract Introduction Knowing a concentration at which cytostatic drugs are toxic for the nasal fibroblasts will enable the use cytostatic drugs in the clinical practice to prevent excessive cicatrization. Objective To determine the cytostatic concentrations of mitomycin С, doxorubicin, and 5-fluorouracil affecting nasal mucosa fibroblasts. Methods We
Int. Arch. Otorhinolaryngol.. Publicado em: 2020-06