Canine Brucellosis
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Soropositividade para Brucella canis: sinais clínicos e fatores associados à infecção em cães atendidos em um centro de diagnóstico por imagem da cidade do Rio de Janeiro. / Seropositivity to Brucella canis: Clinical Signs and Factors Associated with Infection in Dogs Admitted to an Image Diagnosis Center in the City of Rio de Janeiro
The aims of this study were to analyze the clinical signs and factors associated with the seropositivity to Brucella canis in dogs admitted to a veterinary diagnosis center in the city of Rio de Janeiro. Serum samples were collected from 841 dogs, whose participation in the study was allowed by their owners. The samples were analyzed by the Agar Gel Immunodi
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/02/2010
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2. Evaluation of direct and indirect methods of diagnosis of brucellosis in naturally infected dogs / Avaliação de métodos diretos e indiretos de diagnóstico da brucelose em cães naturalmente infectados
Foram comparados procedimentos laboratoriais diretos e indiretos aplicados ao diagnóstico da brucelose canina causada por Brucella canis. Foram examinados 196 cães, os quais foram classificados em infectados, suspeitos e não infectados, de acordo com resultados obtidos no cultivo microbiológico em amostras de sangue, sêmen e swab vaginal, no exame clín
Publicado em: 2006
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3. Soroepidemiologia da brucelose canina causada por Brucella canis e Brucella abortus na cidade de Alfenas, MG
The prevalence of canine brucellosis was evaluated in the city of Alfenas, MG through the technique of agarose gel imunodifusion for Brucella canis and slow serum agglutination test with 2-mercaptoetanol for Brucella abortus. The prevalence was of 14.2% and 2.8%, respectively, for B. canis and B. abortus. The positives, characterized by animals above one yea
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2004-04
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4. Soroprevalência de brucelose canina na cidade de Alfenas, MG: dados preliminares
One hundred and two blood serum samples from dogs referred to the Veterinary Teaching Hospital of Alfenas University, were submitted to the agarose immune diffusion and to the fast serum-agglutination tests addressed to find antibodies anti- Brucella canis and B. abortus, respectively. Five samples (4.9%) were positives for B. canis and none for B. abortus.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2001-06
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5. Diagnostic Usefulness of Antibodies against Ribosome Recycling Factor from Brucella melitensis in Human or Canine Brucellosis
The diagnostic usefulness of an enzyme-linked immunosorbent assay (ELISA) using a purified recombinant ribosome recycling factor from Brucella melitensis (CP24 antigen) was tested in human and canine infections caused by smooth and rough Brucella species, respectively. Anti-CP24 antibodies were detected in 9 (43%) of 21 consecutive cases of canine brucellosi
American Society for Microbiology.
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6. Occurrence and Potential Diagnostic Applications of Serological Cross-Reactivities between Brucella and Other Alpha-Proteobacteria
Agrobacterium, Sinorhizobium, and Ochrobactrum are genera closely related to Brucella but, in contrast to the latter, are not pathogenic for humans and animals. We studied by an indirect enzyme-linked immunosorbent assay (ELISA) the reactivities of brucellosis sera against cytosolic (CYT) and membrane (MA) antigens from these nonpathogenic bacteria, and we e
American Society for Microbiology.
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7. Canine brucellosis: bacteriological and serological investigation of naturally infected dogs in Mexico City.
Bacteriological investigation of canine brucellosis in Mexico City revealed a high rate (11.8%) of Brucella canis infection in a sampling of 59 stray dogs. When conservative criteria were employed in the interpretation of serological test results, there was general agreement between the serological and bacteriological findings; however, some animals with loc
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8. Improved rapid slide agglutination test for presumptive diagnosis of canine brucellosis.
A modified rapid slide agglutination test for the presumptive identification of Brucella canis infection in dogs has been developed. The method required mixing 0.1 ml of canine serum with 0.1 ml of 0.2 M 2-mercaptoethanol solution. Equal volumes (0.05 ml) of the treated serum and the B. canis plate antigen were mixed. Agglutination results were read within 2
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9. Purification of a Brucella canis cell wall antigen by using immunosorbent columns and use of the antigen in enzyme-linked immunosorbent assay for specific diagnosis of canine brucellosis.
A cell wall antigen of Brucella canis was purified by immunosorbent columns. The antigen contained two proteins of 30 and 28 kilodaltons and a polysaccharide exhibiting a 12-kilodalton band upon 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antibody to the purified antigen, which specifically reacted with the polysaccharide, was used as th
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10. Antibody Reactivity to Omp31 from Brucella melitensis in Human and Animal Infections by Smooth and Rough Brucellae
Group 3 of outer membrane proteins (OMPs) of Brucella includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, except Brucella abortus. Antibodies to Brucella melitensis Omp31 have been sought only in infected sheep, and Western blotting of sera from infected sheep did no
American Society for Microbiology.
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11. Brucella abortus infection in indigenous Korean dogs
Three dogs reared on a dairy farm with a high incidence for Brucella abortus were serologically positive for B. abortus and no other Brucella spp. The identity of the organism was confirmed to be B. abortus by AMOS (abortus melitensis ovis suis)-polymerase chain reaction with specific primers for B. canis. One hundred percent homology of the canine isolate a
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12. Comparative Sensitivity of Gel-Diffusion and Tube Agglutination Tests for the Detection of Brucella canis Antibodies in Experimentally Infected Dogs
The microslide gel-diffusion and macro-tube agglutination techniques to detect Brucella canis antibodies in dogs were compared. Sera from dogs experimentally infected with B. canis and a random sample of dog sera with unknown histories of exposure to this organism were examined. The results of the gel-diffusion method employing specific rough Brucella saline