Bovine Oocytes
Mostrando 1-12 de 102 artigos, teses e dissertações.
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1. Dynamics of nuclear and cytoplasm maturation of bovine oocytes cultivated in vitro in medium supplemented with fulerol
RESUMO O objetivo deste estudo foi avaliar, in vitro, a dinâmica da maturação nuclear e citoplasmática de oócitos bovinos em meio MIV tradicional (TC) e suplementado com fulerol (MF50), durante 36 horas. Na maturação nuclear do TC (n=300) e do MF50 (n=270) a cada seis horas, corados com Hoechst 33342, e na citoplasmática, avaliou-se a distribuição
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2022
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2. Oocytes, embryos and pluripotent stem cells from a biomedical perspective
Abstract The veterinary and animal science professions are rapidly developing and their inherent and historical connection to agriculture is challenged by more biomedical and medical directions of research. While some consider this development as a risk of losing identity, it may also be seen as an opportunity for developing further and more sophisticated co
Anim. Reprod.. Publicado em: 28/11/2019
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3. Validation of reference genes for gene expression studies in bovine oocytes and cumulus cells derived from in vitro maturation
Abstract Quantitative real-time PCR (qPCR) is a valuable tool for gene expression studies and it is necessary to choose an ideal endogenous reference gene for data normalization. This work studied a set of reference genes in oocytes and cumulus cells of COCs (Cumulus-Oocyte Complexes) that are suitable for relative gene expression analyses after in vitro mat
Anim. Reprod.. Publicado em: 25/11/2019
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4. Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines
RESUMO O objetivo deste trabalho foi avaliar o efeito do rolipram durante a maturação de oócitos bovinos, expressão gênica e embriões produzidos in vitro. Os ovários bovinos foram coletados no matadouro. Os COCs foram selecionados e divididos em cinco grupos: controle 0 tempo; controle: MIV por 24 horas; tratamentos rolipram com bloqueio MIV por 24 ho
Arq. Bras. Med. Vet. Zootec.. Publicado em: 28/10/2019
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5. Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
ABSTRACT This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro
R. Bras. Zootec.. Publicado em: 30/09/2019
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6. Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
RESUMO O objetivo desse estudo foi avaliar a suplementação de meio de cultura de embriões com antioxidante obtido do extrato oleoso da Lippia origanoides no desenvolvimento e na qualidade de blastocistos produzidos in vitro. Oócitos coletados de ovários de matadouros foram maturados e fertilizados in vitro segundo procedimento laboratorial padrão. Zigo
Arq. Bras. Med. Vet. Zootec.. Publicado em: 14/06/2019
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7. Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In
R. Bras. Zootec.. Publicado em: 2014-03
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8. Use of sex-sorted and unsorted frozen/thawed sperm and in vitro fertilization events in bovine oocytes derived from ultrasound-guided aspiration
The objective was to investigate the effects of sex-sorting on early fertilization events in ovum pick up (OPU) derived oocytes fertilized in vitro with frozen-thawed sperm at different co-incubation lengths. Eighty-four OPU sessions were carried out in 18 cyclic, dry and non-stimulated Holstein Friesian and German black pied cows. Ovum pick up oocytes were
R. Bras. Zootec.. Publicado em: 2013-10
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9. Effects of green tea polyphenols, insulin-like growth factor I and glucose on developmental competence of bovine oocytes
The present study examined the effects of green tea polyphenols (GTP), insulin-like growth factor-I (IGF-I) and glucose on oocyte in vitro maturation, subsequent embryo development and blastocyst quality in bovine. Cumulus-oocyte complexes (COC) were aspirated from the ovaries and cultured in synthetic oviduct fluid supplemented with MEM amino acids (SOFaa)
R. Bras. Zootec.. Publicado em: 2012-12
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10. Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers
The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2) before two treatments: stimula
R. Bras. Zootec.. Publicado em: 2012-12
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11. O óxido nítrico e os nucleotídeos cíclicos em oócitos bovinos maturados in vitro / Nitric oxide and cyclic nucleotides in bovine oocytes matured in vitro
O óxido nítrico (NO) é um mensageiro químico gerado pela atividade da enzima óxido nítrico sintase (NOS) a qual foi detectada em vários órgãos incluído o sistema reprodutor. O sistema NOS/NO parece desempenhar papel importante na maturação oocitária entre outras funções. No entanto, apesar das evidências, há poucos estudos sobre o papel dess
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/09/2011
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12. Vitrificação de ovócitos imaturos de bovinos / Vitrification of bovine immature oocytes
Objetivou-se avaliar o efeito de soluções vitrificantes e da vitrificação em ovócitos imaturos bovino. Os ovócitos foram obtidos a partir de ovários coletados de fêmeas bovinas logo após o abate, selecionados morfologicamente e distribuídos de acordo com os tratamentos. O procedimento experimental foi dividido em duas fases. Na primeira fase foi te
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/08/2011