Bnr
Mostrando 13-19 de 19 artigos, teses e dissertações.
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13. Stable and Dynamic Axes of Polarity Use Distinct Formin Isoforms in Budding YeastD⃞V⃞
Bud growth in yeast is guided by myosin-driven delivery of secretory vesicles from the mother cell to the bud. We find transport occurs along two sets of actin cables assembled by two formin isoforms. The Bnr1p formin assembles cables that radiate from the bud neck into the mother, providing a stable mother-bud axis. These cables also depend on septins at th
The American Society for Cell Biology.
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14. Fusion-mediated transfer of plasmids into Spiroplasma floricola cells.
We have developed and characterized a system for the transfer of plasmids encapsulated in large unilamellar vesicles (LUV) into Spiroplasma floricola BNR1 cells. The approach is based on the ability of S. floricola-derived LUV to fuse with S. floricola cells. The fusion was continuously monitored by an assay for lipid mixing based on the dequenching of the f
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15. Microtubule capture by the cleavage apparatus is required for proper spindle positioning in yeast
Cell division is the result of two major cytoskeletal events: partition of the chromatids by the mitotic spindle and cleavage of the cell by the cytokinetic apparatus. Spatial coordination of these events ensures that each daughter cell inherits a nucleus. Here we show that, in budding yeast, capture and shrinkage of astral microtubules at the bud neck is re
Cold Spring Harbor Laboratory Press.
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16. Arabidopsis Formin AtFH6 Is a Plasma Membrane–Associated Protein Upregulated in Giant Cells Induced by Parasitic Nematodes
Plant-parasitic nematodes Meloidogyne spp induce an elaborate permanent feeding site characterized by the redifferentiation of root cells into multinucleate and hypertrophied giant cells. We have isolated by a promoter trap strategy an Arabidopsis thaliana formin gene, AtFH6, which is upregulated during giant cell formation. Formins are actin-nucleating prot
American Society of Plant Biologists.
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17. Application of the Ovarian Teratoma Mapping Method in the Mouse
Murine ovarian teratomas were used to determine recombination percentages for gene-gene and centromere-gene intervals. Data were obtained utilizing a recombinant inbred strain, LTXBJ, and a number of newly developed LT/SvEi congenic strains. ——Centromere-gene recombination was measured at 11.3 ± 1.2% for the centromere of chromosome 7 – Gpi-1 interval
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18. Cultivation and partial characterization of spiroplasmas in cell cultures.
Spiroplasmas were propagated in the Drosophila melanogaster cell line Dm-1. Spiroplasma citri and unidentified strains (corn shunt organism, 277F [tick isolate], powder puff, BNR-1, honey bee, and OBMG) grew to 10(8) to 10(9) colony-forming units per ml and could be passaged. Cytopathic effect (CPE) varied with the infecting spiroplasma. The honey bee isolat
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19. Dynamic Localization and Function of Bni1p at the Sites of Directed Growth in Saccharomyces cerevisiae
Formin homology (FH) proteins are implicated in cell polarization and cytokinesis through actin organization. There are two FH proteins in the yeast Saccharomyces cerevisiae, Bni1p and Bnr1p. Bni1p physically interacts with Rho family small G proteins (Rho1p and Cdc42p), actin, two actin-binding proteins (profilin and Bud6p), and a polarity protein (Spa2p).
American Society for Microbiology.