Bmal1
Mostrando 13-24 de 28 artigos, teses e dissertações.
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13. New role of zCRY and zPER2 as regulators of sub-cellular distributions of zCLOCK and zBMAL proteins
The core oscillator that generates circadian rhythm in eukaryotes consists of transcription/translation-based autoregulatory feedback loops by which clock gene products negatively regulate their own expression. Control of the accumulation and nuclear entry of the negative regulators PER and CRY is believed to be a key step in these loops. We clarified the mu
Oxford University Press.
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14. Insight into molecular core clock mechanism of embryonic and early postnatal rat suprachiasmatic nucleus
Rhythmicity of the rat suprachiasmatic nucleus (SCN), a site of the circadian clock, develops prenatally. A molecular clockwork responsible for the rhythmicity consists of clock genes and their negative and positive transcriptional–translational feedback loops. The aim of the present study was to discover the development of the clockwork during ontogenesis
National Academy of Sciences.
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15. A noncanonical E-box enhancer drives mouse Period2 circadian oscillations in vivo
The mouse Period2 (mPer2) locus is an essential negative-feedback element of the mammalian circadian-clock mechanism. Recent work has shown that mPer2 circadian gene expression persists in both central and peripheral tissues. Here, we analyze the mouse mPer2 promoter and identify a circadian enhancer (E2) with a noncanonical 5′-CACGTT-3′ E-box located 20
National Academy of Sciences.
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16. Circadian sensitivity to the chemotherapeutic agent cyclophosphamide depends on the functional status of the CLOCK/BMAL1 transactivation complex
The circadian clock controls many aspects of mammalian physiology, including responses to cancer therapy. We find that wild-type and circadian mutant mice demonstrate striking differences in their response to the anticancer drug cyclophosphamide (CY). While the sensitivity of wild-type mice varies greatly, depending on the time of drug administration, Clock
National Academy of Sciences.
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17. Toward a detailed computational model for the mammalian circadian clock
We present a computational model for the mammalian circadian clock based on the intertwined positive and negative regulatory loops involving the Per, Cry, Bmal1, Clock, and Rev-Erb α genes. In agreement with experimental observations, the model can give rise to sustained circadian oscillations in continuous darkness, characterized by an antiphase relat
National Academy of Sciences.
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18. Circadian rhythm transcription factor CLOCK regulates the transcriptional activity of the glucocorticoid receptor by acetylating its hinge region lysine cluster: potential physiological implications
Glucocorticoids, end products of the hypothalamic-pituitary-adrenal axis, influence functions of virtually all organs and tissues through the glucocorticoid receptor (GR). Circulating levels of glucocorticoids fluctuate naturally in a circadian fashion and regulate the transcriptional activity of GR in target tissues. The basic helix-loop-helix protein CLOCK
The Federation of American Societies for Experimental Biology.
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19. Targeted Disruption of the mPer3 Gene: Subtle Effects on Circadian Clock Function
Neurons in the mammalian suprachiasmatic nucleus (SCN) contain a cell-autonomous circadian clock that is based on a transcriptional-translational feedback loop. The basic helix-loop-helix–PAS proteins CLOCK and BMAL1 are positive regulators and drive the expression of the negative regulators CRY1 and CRY2, as well as PER1, PER2, and PER3. To assess the rol
American Society for Microbiology.
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20. Bimodal regulation of mPeriod promoters by CREB-dependent signaling and CLOCK/BMAL1 activity
Circadian rhythmicity in mammals is under the control of a molecular pacemaker constituted of clock gene products organized in transcriptional autoregulatory loops. Phase resetting of the clock in response to light involves dynamic changes in the expression of several clock genes. The molecular pathways used by light to influence pacemaker-driven oscillation
The National Academy of Sciences.
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21. Role of DBP in the Circadian Oscillatory Mechanism
Transcript levels of DBP, a member of the PAR leucine zipper transcription factor family, exhibit a robust rhythm in suprachiasmatic nuclei, the mammalian circadian center. Here we report that DBP is able to activate the promoter of a putative clock oscillating gene, mPer1, by directly binding to the mPer1 promoter. The mPer1 promoter is cooperatively activa
American Society for Microbiology.
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22. Temporal expression of seven clock genes in the suprachiasmatic nucleus and the pars tuberalis of the sheep: Evidence for an internal coincidence timer
The 24-h expression of seven clock genes (Bmal1, Clock, Per1, Per2, Cry1, Cry2, and CK1ɛ) was assayed by in situ hybridization in the suprachiasmatic nucleus (SCN) and the pars tuberalis (PT) of the pituitary gland, collected every 4 h throughout 24 h, from female Soay sheep kept under long (16-h light/8-h dark) or short (8-h light/16-h dark) photoperiods.
The National Academy of Sciences.
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23. Control of Intracellular Dynamics of Mammalian Period Proteins by Casein Kinase I ɛ (CKIɛ) and CKIδ in Cultured Cells
Recent studies have shown that casein kinase I ɛ (CKIɛ) is an essential regulator of the mammalian circadian clock. However, the detailed mechanisms by which CKIɛ regulates each component of the circadian negative-feedback loop have not been fully defined. We show here that mPer proteins, negative limbs of the autoregulatory loop, are specific substrates
American Society for Microbiology.
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24. Circadian Regulation of nocturnin Transcription by Phosphorylated CREB in Xenopus Retinal Photoreceptor Cells
Although CLOCK/BMAL1 heterodimers have been implicated in transcriptional regulation of several rhythmic genes in vitro through E-box sequence elements, little is known about how the circadian clock regulates rhythmic genes with diverse phases in vivo. The gene nocturnin is rhythmically transcribed in Xenopus retinal photoreceptor cells, which contain endoge
American Society for Microbiology.