Blastocyst
Mostrando 13-24 de 245 artigos, teses e dissertações.
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13. Genes Regulating Maternal Recognition of Pregnancy in Domestic Animals: an Update
ABSTRACT Early embryonic mortality is one of the main sources of reproductive wastages and major constraints for full exploitation of the production potential of livestock. The survivality of embryo during early embryonic life is mostly dependent on the efficiency with which the maternal recognition of pregnancy (MRP) is established. Maternal recognition of
Braz. arch. biol. technol.. Publicado em: 2015-12
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14. Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In
R. Bras. Zootec.. Publicado em: 2014-03
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15. CARACTERIZAÇÃO MOLECULAR DOS COMPONENTES DO SISTEMA ANGIOTENSINA-(1-7) DURANTE A DIVERGÊNCIA FOLICULAR E EXPRESSÃO DE GENES DE REPARO DA FITA DUPLA DE DNA EM EMBRIÕES BOVINOS / MOLECULAR CHARACTERIZATION OF THE ANGIOTENSIN-(1-7) SYSTEM COMPONENTS DURING FOLLICULAR DEVIATION AND EXPRESSION OF DNA DOUBLE-STRANDED REPAIR GENES IN BOVINE EMBRYOS
The first study characterized the expression of MAS receptor and key enzymes for Ang-(1-7) production, such as, ACE2, NEP and PEP during follicular development. Furthermore, the regulation of local Ang1-7 system was evaluated after the intrafollicular injection of fulvestrant (an estradiolreceptor inhibitor) in the dominant follicle. Cows were ovariectomized
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/02/2012
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16. Effects of green tea polyphenols, insulin-like growth factor I and glucose on developmental competence of bovine oocytes
The present study examined the effects of green tea polyphenols (GTP), insulin-like growth factor-I (IGF-I) and glucose on oocyte in vitro maturation, subsequent embryo development and blastocyst quality in bovine. Cumulus-oocyte complexes (COC) were aspirated from the ovaries and cultured in synthetic oviduct fluid supplemented with MEM amino acids (SOFaa)
R. Bras. Zootec.. Publicado em: 2012-12
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17. Avaliação do swim-up e do gradiente descontinuo de densidade na pré-seleção do sexo de espermatozoides na produção de embriões bovinos
The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validate
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2012-06
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18. Avaliação da Proporção Sexual de Embriões Desenvolvidos In Vitro e de Progênie a Campo de Touros Jovens. / Evaluation of the Sex Ratio of Embryos Developed In Vitro and in Field Progeny of Young Sires.
Some of the main problems that affect the in vitro production of embryos is the variation among bulls in relation to fertility and the greater birth of male embryos. Many factors can change the ratio 1:1 between the genders, both in the in vitro production of embryos and in the method of artificial insemination. To try to change this sex ratio in vitro, we s
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/08/2011
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19. The effects of ovalbumin as a protein source during the in vitro production of bovine embryos
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 1
Revista Brasileira de Zootecnia. Publicado em: 2011-10
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20. Modulation of murine blastocyst hatching in vitro by glutamine and tryptophan
Enrichment of culture media with amino acids improves embryo development. However, little is known about the specific action of each amino acid during embryogenesis. The present study was undertaken to examine the effect of L-glutamine (Gln) and tryptophan (Trp) on mouse embryo hatching, expansion and viability in vitro. Blastocysts were collected from 6- to
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-08
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21. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with io
Animal Reproduction Science. Publicado em: 2011
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22. Development of bovine embryos cultured with media containing glucose.
Blastocyst (BL) expansion and blastocoel formation are coupled with increase in the glucose (GLU) metabolism, which is higher in morphologically normal than in degenerate embryos (Rieger et aI., 1992). This study aimed to determine the dose levei and duration of addition of GLU to culture medium that ensures the best results in development of in vitro produc
CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL. Publicado em: 2011
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23. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments.
In vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (I) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of
Zygote. Publicado em: 2011
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24. Nova técnica para contagem do número de células de blastocistos
A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture, the blastococysts were selected to test the new proposal of
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2010-12