Bis Ans
Mostrando 1-11 de 11 artigos, teses e dissertações.
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1. PurificaÃÃo, caracterizaÃÃo de uma lectina e compostos fenÃlicos da entrecasca de Sebastiania jacobinensis (Muill. Arg.): efeito de radiaÃÃo gama sobre estrutura-atividade protÃica e proteÃÃo de flavonÃides isolados
A lectin from Sebastiania jacobinensis bark (SejaBL) was isolated using a combination of acetone precipitation, fractionation by ammonium sulphate, ion exchange and gel filtration chromatographies. The molecular mass of SejaBL was determined as approximately 52.0kDa by SDS-PAGE and 50.0kDa by gel filtration. The lectin is a glycoprotein with a neutral carboh
Publicado em: 2009
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2. A fluorescence probe study of gemini surfactants in aqueous solution: a comparison between n-2-n and n-6-n series of the alkanediyl-a,w-bis (dimethylalkylammonium bromides)
Two series of alkanediyl-a,w-bis (dimethylalkylammonium bromide (n-2-n and n-6-n; n=8, 10,12, and 16) have been synthesized and their micelles properties studied in aqueous solution using pyrene, pyrenecarboxaldehyde (PCA) and 1,8 anilinonaphtalene sulfonic acid sodium salt (ANS) as fluorescent probes. The micelles from these surfactants have been characteri
Eclética Química. Publicado em: 2007
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3. Estudos de enovelamento da Isoforma 1 da Lectina de Sementes deCratylia mollis : caracterizaÃÃo de estados intermediÃrios
AlÃm do de interesse acadÃmico, o conhecimento acerca do enovelamento de proteÃnas à empregado em muitas aplicaÃÃes biotecnolÃgicas com importÃncia industrial. Lectinas sÃo proteÃnas capazes reconhecer com especificidade diferentes sacarÃdeos, estando envolvidas em uma variedade processos biolÃgicos. Diversos modelos de associaÃÃes quaternÃria
Publicado em: 2006
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4. Efeito de alta pressão hidrostatica e baixa temperatura no virus do mosaico dotabaco (TMV)
Investigou-se o efeito de baixa temperatura e diferentes concentrações de uréia combinados a alta pressão hidrostática (até 2,5 kbar) no Vírus do Mosaico do Tabaco (TMV), um vírus cilíndrico com 40.000 kDa de massa molecular, composto de aproximadamente 2.130 cópias da proteína capsídica (17,5 kDa) envolvendo uma fita de RNA positiva. O vírus fo
Publicado em: 1996
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5. A Small Molecule Inhibits and Misdirects Assembly of Hepatitis B Virus Capsids
Hepatitis B virus (HBV) capsids play an important role in viral nucleic acid metabolism and other elements of the virus life cycle. Misdirection of capsid assembly (leading to formation of aberrant particles) may be a powerful approach to interfere with virus production. HBV capsids can be assembled in vitro from the dimeric capsid protein. We show that a sm
American Society for Microbiology.
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6. 4,4'-Bis (1-anilinonaphthalene 8-sulfonate) (bis-ANS): a new probe of the active site of myosin.
The interaction of myosin subfragment-1 (S-1) with 4,4'-bis(1-anilinonaphthalene 8-sulfonate) (bis-ANS) has been studied by monitoring the fluorescence of the latter when the two components form a complex. Because ATP and ATP analogs partially displace complexed bis-ANS it has also been possible to study interactions of S-1 and nucleotides by using the displ
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7. Application of Fluorescence Spectroscopy to Quantify Shear-Induced Protein Conformation Change
Rapid and robust methods are required to quantify the effect of hydrodynamic shear on protein conformation change. We evaluated such strategies in this work and found that the binding of the fluorescent probe 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid (bis-ANS) to hydrophobic pockets in the blood protein von Willebrand factor (VWF) is enhanced
The Biophysical Society.
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8. Kinetic and Thermodynamic Characterization of Dihydrotestosterone-Induced Conformational Perturbations in Androgen Receptor Ligand-Binding Domain
Ligand-induced conformational perturbations in androgen receptor (AR) are important in coactivator recruitment and transactivation. However, molecular rearrangements in AR ligand-binding domain (AR-LBD) associated with agonist binding and their kinetic and thermodynamic parameters are poorly understood. We used steady-state second-derivative absorption and e
The Endocrine Society.
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9. Structural Characterization of Clusterin-Chaperone Client Protein Complexes*
Clusterin (CLU) is a potent extracellular chaperone that inhibits protein aggregation and precipitation otherwise caused by physical or chemical stresses (e.g. heat, reduction). This action involves CLU forming soluble high molecular weight (HMW) complexes with the client protein. Other than their unquantified large size, the physical characteristics of thes
American Society for Biochemistry and Molecular Biology.
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10. A redox-sensitive loop regulates plasminogen activator inhibitor type 2 (PAI-2) polymerization
Plasminogen activator inhibitor type 2 (PAI-2) is the only wild-type serpin that polymerizes spontaneously under physiological conditions. We show that PAI-2 loses its ability to polymerize following reduction of thiol groups, suggesting that an intramolecular disulfide bond is essential for the polymerization. A novel disulfide bond was identified between C
Oxford University Press.
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11. Thermally denatured state determines refolding in lipase: Mutational analysis
Irreversibility of thermally denatured proteins due to aggregation limits thermodynamic characterization of proteins and also confounds the identification of thermostable mutants in protein populations. Identification of mutations that prevent the aggregation of unfolded proteins provides insights into folding pathways. In a lipase from Bacillus subtilis, ev
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