Barrel Stave
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Análise experimental de aduelas pré-moldadas em concreto de alto desempenho para passarelas estaiadas / Theoretical and experimental analysis of precast barrel staves in high performance concrete
Embora a utilização do concreto de alto desempenho (CAD) para a fabricação de elementos pré-moldados seja frequentemente usado na construção civil, o presente trabalho apresenta um estudo do emprego de microconcreto em aduelas tubulares de pequenas espessuras protendidas dentro de critérios fabris, com materiais regionais, a fim de repassar para a in
Publicado em: 2008
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2. Estudo computacional da interação entre bicamada lipídica aniônica e moricina
Neste trabalho foram desenvolvidas cinco simulações: Moricina no vácuo; moricina em meio fisiológico (água e íons); membrana em meio fisiológico; 1 moricina e 6 moricinas no interior da membrana. O objetivo é avaliar as interações antibiótico – membrana. Para esse estudo foi escolhido um peptídeo catiônico com atividade bactericida já constat
Publicado em: 2007
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3. Transmembrane pores formed by synthetic p-octiphenyl β-barrels with internal carboxylate clusters: Regulation of ion transport by pH and Mg2+- complexed 8-aminonaphthalene-1,3,6-trisulfonate
Design, synthesis, and study of a synthetic barrel-stave supramolecule with p-octiphenyl “staves,” β-sheet “hoops,” and hydrophobic exterior as well as internal carboxylate clusters are reported. Ion transport experiments indicate the formation of transmembrane pores at 5 < pH < 7 with nanomolar activity. Blockage of dye efflux from spherical bilaye
National Academy of Sciences.
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4. Assembly of the Mitochondrial Apoptosis-induced Channel, MAC*S⃞
Although Bcl-2 family proteins control intrinsic apoptosis, the mechanisms underlying this regulation are incompletely understood. Patch clamp studies of mitochondria isolated from cells deficient in one or both of the pro-apoptotic proteins Bax and Bak show that at least one of the proteins must be present for formation of the cytochrome c-translocating
American Society for Biochemistry and Molecular Biology.
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5. Two classes of alamethicin transmembrane channels: molecular models from single-channel properties.
Molecular structures of transmembrane channels formed by alamethicin polypeptide aggregates were analyzed by measuring open-channel conductances and state-transition kinetics using voltage-clamp technique with artificial phospholipid bilayers isolated onto micropipettes by a novel solvent-free tip-dip method. Two distinct classes of alamethicin channels, eac
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6. Isolation, partial characterization, and mode of action of Acidocin J1132, a two-component bacteriocin produced by Lactobacillus acidophilus JCM 1132.
Lactobacillus acidophilus JCM 1132 produces a heat-stable, two-component bacteriocin designated acidocin J1132 that has a narrow inhibitory spectrum. Maximum production of acidocin J1132 in MRS broth was detected at pH 5.0. Acidocin J1132 was purified by ammonium sulfate precipitation and sequential cation exchange and reversed-phase chromatographies. Acidoc
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7. Neutron scattering in the plane of membranes: structure of alamethicin pores.
A technique of neutron in-plane scattering for studying the structures of peptide pores in membranes is described. Alamethicin in the inserted state was prepared and undeuterated and deuterated dilauroyl phosphatidylcholine (DLPC) hydrated with D2O or H2O. Neutron in-plane scattering showed a strong dependence on deuteration, clearly indicating that water is
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8. Influence of proline position upon the ion channel activity of alamethicin.
Alamethicin, a 20-residue peptaibol, induces voltage-dependent ion channels in lipid bilayers according to the barrel-stave model. To study relationships between the proline-14-induced kink region and the channel-forming behavior of the peptide, a set of alamethicin analogs with proline incorporated at positions 11, 12, 13, 14, 15, 16, and 17, respectively,
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9. A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides.
A lactococcal bacteriocin, termed lactococcin G, was purified to homogeneity by a simple four-step purification procedure that includes ammonium sulfate precipitation, binding to a cation exchanger and octyl-Sepharose CL-4B, and reverse-phase chromatography. The final yield was about 20%, and nearly a 7,000-fold increase in the specific activity was obtained