Baculovirus Replication
Mostrando 1-12 de 159 artigos, teses e dissertações.
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1. Biologia molecular de baculovirus e seu uso no controle biologico de pragas no Brasil.
Os baculovirus sao virus patogenicos a insetos, encontrados principalmente na ordem Lepidoptera. A familia Baculoviridae e taxonomicamente dividida em dois generos: Nucleopolyhedrovirus e Granulovirus, que diferem pela morfologia do corpo de oclusao. Os nucleopoliedrovirus (NPV) possuem corpos de inclusao poliedrica (PIB), contendo multiplas particulas virai
Pesquisa Agropecuaria Brasileira. Publicado em: 2011
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2. Molecular cloning and expression of key gene encoding hypothetical DNA polymerase from B. mori parvo-like virus
BmPLV-Z is the abbreviation for Bombyx mori parvo-like virus (China isolate). This is a novel virus with two single-stranded linear DNA molecules, viz., VD1 (6543 bp) and VD2 (6022 bp), which are encapsidated respectively into separate virions. Analysis of the deduced amino acid sequence of VD1-ORF4 indicated the existence of a putative DNA-polymerase with e
Genetics and Molecular Biology. Publicado em: 15/10/2010
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3. Caracterização genômica da bactéria endossimbiótica do tripanosomatídeo Crithidia deanei e de suas DNA topoisomerases / Genomics characterization of the endosymbiont bacterium of the tripanosomatídeo Crithidia deanei and of its DNA topoisomerases
The present work is focused in the endosymbiont type II topoisomerases, due to their essential roles not only in the replication and transcription processes but also at the recombination and chromosome segregation processes. There are two Type II topoisomerases in bacteria: DNA gyrase and DNA Topoisomerase IV. DNA gyrase is the only topoisomerase capable of
Publicado em: 2006
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4. Baculovirus replication in a mosquito (dipteran) cell line.
The baculovirus from the lepidopteran host Autographa californica (alfalfa looper) was shown to replicate in a dipteran cell line without the production of characteristic polyhedral inclusion bodies. The low level of replication could not be detected by 50% tissue culture infective dose titrations, but was apparent by [3H]thymidine labeling of the viral geno
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5. Characterization of the Interaction between P143 and LEF-3 from Two Different Baculovirus Species: Choristoneura fumiferana Nucleopolyhedrovirus LEF-3 Can Complement Autographa californica Nucleopolyhedrovirus LEF-3 in Supporting DNA Replication
The baculovirus protein P143 is essential for viral DNA replication in vivo, likely as a DNA helicase. We have demonstrated that another viral protein, LEF-3, first described as a single-stranded DNA binding protein, is required for transporting P143 into the nuclei of insect cells. Both of these proteins, along with several other early viral proteins, are a
American Society for Microbiology.
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6. Replication, Integration, and Packaging of Plasmid DNA following Cotransfection with Baculovirus Viral DNA
Infection-dependent replication assays have been used to identify numerous putative origins of baculovirus replication. However, plasmid DNA, when cotransfected into insect cells with Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) DNA, replicates independently of any viral sequence in cis (11). Cotransfection of transfer plasmids and
American Society for Microbiology.
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7. A new method for the isolation of recombinant baculovirus.
An improved method for the isolation of baculovirus recombinants is described. The method involves the replication and maintenance of the baculovirus genome in the yeast Saccharomyces cerevisiae which was accomplished by the isolation of a baculovirus recombinant containing yeast ARS and CEN sequences ensuring stable replication in yeast and a URA3 selectabl
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8. Identification of genes involved in DNA replication of the Autographa californica baculovirus.
By use of a transient replication assay, nine genes involved in DNA replication were identified in the genome of the Autographa californica baculovirus. Six genes encoding helicase, DNA polymerase, IE-1, LEF-1, LEF-2, and LEF-3 are essential for DNA replication while three genes encoding P35, IE-2, and PE38 stimulate DNA replication. No stimulation by the Ac
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9. Use of the Hepatitis B Virus Recombinant Baculovirus-HepG2 System to Study the Effects of (−)-β-2′,3′-Dideoxy-3′-Thiacytidine on Replication of Hepatitis B Virus and Accumulation of Covalently Closed Circular DNA
(−)-β-2′,3′-Dideoxy-3′-thiacytidine (lamivudine [3TC]) is a nucleoside analog which effectively interferes with the replication of hepatitis B virus (HBV) DNA in vitro and in vivo. We have investigated the antiviral properties of 3TC in vitro in HepG2 cells infected with recombinant HBV baculovirus. Different types of information can be obtained wit
American Society for Microbiology.
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10. Reconstitution of human replication factor C from its five subunits in baculovirus-infected insect cells
Human replication factor C (RFC, also called activator 1) is a five-subunit protein complex (p140, p40, p38, p37, and p36) required for proliferating cell nuclear antigen (PCNA)-dependent processive DNA synthesis catalyzed by DNA polymerase δ or ɛ. Here we report the reconstitution of the RFC complex from its five subunits simultaneously overexpressed
The National Academy of Sciences of the USA.
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11. Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus.
Sequences encoding adenovirus type 2 DNA polymerase were placed under control of the polyhedrin promoter and inserted into the baculovirus Autographa californica nuclear polyhedrosis virus by homologous recombination. Insect cells infected with the recombinant virus produced substantial amounts of the adenovirus type 2 DNA polymerase protein which was functi
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12. Baculovirus DNA Replication-Specific Expression Factors Trigger Apoptosis and Shutoff of Host Protein Synthesis during Infection▿
Apoptosis is an important antivirus defense. To define the poorly understood pathways by which invertebrates respond to viruses by inducing apoptosis, we have identified replication events that trigger apoptosis in baculovirus-infected cells. We used RNA silencing to ablate factors required for multiplication of Autographa californica multicapsid nucleopolyh
American Society for Microbiology (ASM).