Bacterial Typing Technique
Mostrando 1-12 de 24 artigos, teses e dissertações.
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1. Molecular analysis of fecal microbiota from healthy newborns / Análise molecular da microbiota fecal de recém-nascidos saudáveis
Purpose: To evaluate by molecular methodology the fecal microbiota of healthy newborns, exclusively breastfed. Materials and methods: Fecal samples from ten neonates were analyzed on 2nd, 7th and 30th day of life, using 16S rDNA sequencing and real-time PCR for bifidobacteria. Results: The fecal bacteria diversity increased from the second to the 30th day of
Publicado em: 2008
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2. Preliminary evaluation of adherence on abiotic and cellular surfaces of Acinetobacter baumannii strains isolated from catheter tips
The cell surface hydrophobicity and adhesion to abiotic and cellular surfaces was tested in five clinical strains of Acinetobacter baumannii isolated from catheter tips. Biochemical and molecular characteristics of these strains were also studied. Hydrophobicity was characterized by a test for affinity to xylene. Adhesion to abiotic surfaces (polystyrene, fo
Brazilian Journal of Infectious Diseases. Publicado em: 2006-10
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3. Molecular characterization of Leptospira sp. strains isolated from human subjects in São Paulo, Brazil using a polymerase chain reaction-based assay: a public health tool
A polymerase chain reaction (PCR)-based assay which amplifies repetitive DNA elements present within bacterial genomes was used to characterize and differentiate Leptospira sp. Thirty-five strains from a reference culture collection and 18 clinical isolates which had been previously analyzed by cross agglutinin absorption test (CAAT) were evaluated by this t
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-06
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4. Avaliação da influência do tratamento diretamente observado (DOT) com e sem profilaxia na transmissão da tuberculose em centros médicos de saúde do município do Rio de Janeiro, utilizando técnicas de tipagem molecular em cepas de M. tuberculosis / Evaluation influence of the treatment directly observed (DOT) with and without Prophylaxis in the transmission of the tuberculosis in medical centers of health of the city of Rio De Janeiro, using tuberculosis techniques of molecular typing in cepas of M. tuberculosis
Tuberculosis (TB) is a major cause of morbidity and mortality worldwide, particularly in developing countries. In the city of Rio de Janeiro, 7,000 new TB cases are reported annually, for an incidence de 105 per 100,000 inhabitants. In three Community Health Centers (CHCs) of Planning Area 1 (AP-1.0) of Rio de Janeiro, the section of the city with the highes
Publicado em: 2006
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5. New Method for Typing Staphylococcus aureus Strains: Multiple-Locus Variable-Number Tandem Repeat Analysis of Polymorphism and Genetic Relationships of Clinical Isolates
The PCR-based methodology applied to multiple-locus variable numbers of tandem repeat (VNTR) analysis was recently shown to be a useful technique for the molecular typing of clinical isolates of several bacterial species. We have adopted this method for the molecular typing of methicillin-resistant Staphylococcus aureus. Five staphylococcal VNTR loci (sdr, c
American Society for Microbiology.
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6. Bacteriocin typing by leakage of ultraviolet light-absorbing material.
A rapid and reproducible method of bacteriocin typing is described based on leakage of ultraviolet light-absorbing material (UVAM), detectable in supernatants of bacteriocin-sensitive cultures, by means of a spectrophotometer. The prerequisites for reproducible results, with nonsignificant fluctuations in standard error of the mean, are: a set of standardize
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7. Use of PCR to study epidemiology of Serratia marcescens isolates in nosocomial infection.
A method to characterize strains of Serratia marcescens based on the PCR amplification of enterobacterial repetitive intergenic consensus sequences has been developed. The PCR fingerprints were generated from boiled supernatants prepared directly from bacterial colonies without the need for DNA extraction. The technique was applied to isolates obtained durin
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8. Multilocus Sequence Typing Is a Reliable Alternative Method to DNA Fingerprinting for Discriminating among Strains of Candida albicans
Multilocus sequence typing (MLST) has emerged as a powerful new DNA-typing tool for the evaluation of intraspecies genetic relatedness. This method relies on DNA sequence analysis of nucleotide polymorphisms in housekeeping genes and has shown a high degree of intraspecies discriminatory power for bacterial and fungal pathogens. However, the results of the M
American Society for Microbiology.
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9. A PCR-based DNA fingerprinting technique: AFLP for molecular typing of bacteria.
Amplified restriction fragment polymorphism (AFLP) is a PCR-based DNA fingerprinting technique. In AFLP analysis, bacterial genomic DNA is digested with restriction enzymes, ligated to adapters, and a subset of DNA fragments are amplified using primers containing 16 adapter defined sequences with one additional arbitrary nucleotide. Polymorphisms of differen
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10. Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods.
Shigella sonnei is a major cause of diarrheal disease in developed as well as in developing countries. Epidemiologic studies of this organism have been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared different molecular typing methods, i.e., plasmid profile analysis, restriction endonuclease analysi
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11. Epidemiological typing of isolates from an outbreak of infection with multidrug-resistant Enterobacter cloacae by repetitive extragenic palindromic unit b1-primed PCR and pulsed-field gel electrophoresis.
An outbreak of multidrug-resistant Enterobacter cloacae infection lasted for 4 months in a neonatal intensive care unit (NICU). Forty-six isolates from the NICU and 20 epidemiologically unrelated strains were characterized by pulsed-field gel electrophoresis (PFGE) and repetitive extragenic palindromic unit b1-primed PCR (REPUb1-PCR) typing. The PFGE pattern
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12. Genomic DNA fingerprinting by restriction fragment end labeling.
A typing method for bacteria was developed and applied to several species, including Escherichia coli and Actinobacillus actinomycetemcomitans. Total genomic DNA was digested with a restriction endonuclease, and fragments were enabled with [alpha-32P]dATP by using the Klenow fragment of DNA polymerase and separated by electrophoresis in 6% polyacrylamide/8 M