Atp Diphosphohydrolase
Mostrando 1-12 de 23 artigos, teses e dissertações.
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1. IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase
Abstract INTRODUCTION: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins. METHODS: Potato apyrase was employed using ELISA, western blot, and mouse immu
Rev. Soc. Bras. Med. Trop.. Publicado em: 28/03/2019
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2. ATIVIDADE DE ECTOENZIMAS E DE COLINESTERASES E BIOMARCADORES DE ESTRESSE OXIDATIVO EM PACIENTES COM CÂNCER DE PULMÃO / ACTIVITY OF ECTOENZIMAS AND CHOLINESTERASES AND OXIDATIVE STRESS BIOMARKERS IN PATIENTS WITH LUNG CANCER
Lung cancer has become one of the most commonly diagnosed neoplastic diseases, as well as one of the most lethal worldwide, characterized, therefore, as a public health problem. This spread of lung cancer in the world population, which is clearly seen in statistics, is closely related to smoking, and smoking is considered one of the main risk factors that le
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/03/2012
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3. Analysis of the NTPDase and ecto-5'-nucleotidase profiles in serum-limited Trichomonas vaginalis
Trichomonas vaginalis is a parasite of the human urogenital tract that causes trichomonosis, the most prevalent non-viral sexually transmitted disease. Ectonucleoside triphosphate diphosphohydrolase (NTPDase) family members, which hydrolyse extracellular ATP and ADP and ecto-5′-nucleotidase, which hydrolyses AMP, have been characterised in T. vaginalis. Fo
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-03
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4. Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg
A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, a
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-11
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5. Gadolinium increases the vascular reactivity of rat aortic rings
Gadolinium (Gd) blocks intra- and extracellular ATP hydrolysis. We determined whether Gd affects vascular reactivity to contractile responses to phenylephrine (PHE) by blocking aortic ectonucleoside triphosphate diphosphohydrolase (E-NTPDase). Wistar rats of both sexes (260-300 g, 23 females, 7 males) were used. Experiments were performed before and after in
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-05
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6. Heterogeneity in extracellular nucleotide hydrolysis among clinical isolates of Trichomonas vaginalis
Trichomonas vaginalis is a parasitic protozoan that causes trichomonosis, a sexually-transmitted disease, with serious sequelae to women and men. As the host–parasite relationship is complex, it is important to investigate biochemical aspects of the parasite that contribute to our understanding of trichomonal biology and pathogenesis. Nucleoside triphospha
Publicado em: 2011
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7. Identificação de alvos antigênicos na ATP difosfohidrolase solúvel de Schistosoma mansoni e possível aplicação funcional de peptídeos sintéticos
The antigenicity of both soluble S. mansoni ATP diphosphohydrolase and membraneassociated isoforms has been recently described. Cross-immunoreactivity between potato apyrase and IgG antibody from S. mansoni-infected mice or schistosomiasis patients strongly suggested that the epitopes shared between the vegetable and parasite proteins are antigenic. By bioin
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/06/2010
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8. Antibody reactivity against potato apyrase, a protein that shares epitopes with Schistosoma mansoni ATP diphosphohydrolase isoforms, in acute and chronically infected mice, after chemotherapy and reinfection
Schistosoma mansoni ATP diphosphohydrolase isoforms and potato apyrase share conserved epitopes. By enzyme-linked immunosorbent assays, elevated levels of IgM, IgG2a and IgG1 antibody reactivity against potato apyrase were observed in S. mansoni-infected BALB/c mice during the acute phase of infection, while only IgM and IgG1 antibody reactivity levels maint
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-07
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9. Detection of IgG1 and IgG4 subtypes reactive against potato apyrase in schistosomiasis patients
In this paper, we showed for the first time that the conserved domains within Schistosoma mansoni ATP diphosphohydrolase isoforms, shared with potato apyrase, possess epitopes for the IgG1 and IgG4 subtypes, as 24 (80%) of the 30 schistosomiasis patients were seropositive for this vegetable protein. The analyses for each patient cured (n = 14) after treatmen
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-07
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10. Brain ischemia alters platelet ATP disphosphohydrolase and 5'-nucleotidase activities in naive and preconditioned rats
The effects of transient forebrain ischemia, reperfusion and ischemic preconditioning on rat blood platelet ATP diphosphohydrolase and 5'- nucleotidase activities were evaluated. Adult Wistar rats were submitted to 2 or 10 min of single ischemic episodes, or to 10 min of ischemia 1 day after a 2-min ischemic episode (ischemic preconditioning) by the four-ves
Publicado em: 2010
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11. Ectonucleotidase activities in Sertoli cells from immature rats
Sertoli cells have been shown to be targets for extracellular purines such as ATP and adenosine. These purines evoke responses in Sertoli cells through two subtypes of purinoreceptors, P2Y2 and PA1. The signals to purinoreceptors are usually terminated by the action of ectonucleotidases. To demonstrate these enzymatic activities, we cultured rat Sertoli cell
Publicado em: 2010
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12. ATP DIFOSFOHIDROLASE EM FORMAS PROMASTIGOTAS DE Leishmania (Viannia) braziliensis: CARACTERIZAÇÃO E ANÁLISE DE DOMÍNIOS COMPARTILHADOS COM A APIRASE DE Solanum tuberosum
Uma ATP difosfohidrolase foi identificada em promastigotas de Leishmania braziliensis, e resultado similar foi encontrado em outra cepa de Leishmania, pertencente ao Complexo Leishmania braziliensis. Hidrólise de nucleosídeos di- e trifosfatados sob estímulo de íons bivalentes, associada à inibição por azida sódica, sugeriram que atividade ATP difosf
Publicado em: 2007