Asymmetry Measurements
Mostrando 25-36 de 40 artigos, teses e dissertações.
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25. Transcriptional measurements of mouse repeated DNA sequences.
We have carried out transcriptional measurements on several families of repeated sequences to define their expression in mouse cells. The majority of Alu family transcripts result from read-through from adjacent structural gene promoters while 20% are discrete RNA polymerase III products. Alu repeat members show preferential orientation within RNA polymerase
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26. High resolution SPECT with [99mTc]-d,l-HMPAO in normal pressure hydrocephalus before and after shunt operation.
Cranial CT and high resolution measurements of regional cerebral blood flow (rCBF) with brain dedicated single photon emission computer tomography (SPECT) and [99mTc]-d,l-hexamethylpropyleneamine oxime ([99mTc]-d,l-HMPAO) were performed before and after shunt operation in 14 consecutive patients with dementia and normal pressure hydrocephalus (NPH). When com
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27. Limitations of circulation time in the diagnosis of intracranial disease1
The mode circulation time was measured using intravenously injected technetium 99m and a collimation system devised to discriminate between the right and left sides of the head. The results in 21 normal men were used as a basis of assessing the findings in 205 patients, made up of five diagnostic groups (ischaemia, haematoma, subarachnoid haemorrhage, intrac
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28. A description of activation and conduction in calcium channels based on tail and turn-on current measurements in the snail.
Turn-on of Ca currents, or activation, was compared with turn-off, or deactivation. The experiments were done on nerve cell bodies of Helix aspersa separated by dissection, voltage-clamped and internally perfused using a combined suction pipette--micro-electrode method. Ca currents were isolated by suppression of Na and K currents. The turn-off or tail curre
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29. Structural analysis at 2.2 Å of orthorhombic crystals presents the asymmetry of the allophycocyanin–linker complex, AP⋅LC7.8, from phycobilisomes of Mastigocladus laminosus
An electrophoretically purified allophycocyanin–linker complex, AP⋅LC7.8, from phycobilisomes of Mastigocladus laminosus has been crystallized in the orthorhombic space group P212121. Cryocrystallographic x-ray measurements enabled the structural analysis of the complex at a resolution of 2.2 Å. The asymmetric unit contains two side-to-side assoc
The National Academy of Sciences.
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30. In Vitro Low-Level Resistance to Azoles in Candida albicans Is Associated with Changes in Membrane Lipid Fluidity and Asymmetry
The present study tracks the development of low-level azole resistance in in vitro fluconazole-adapted strains of Candida albicans, which were obtained by serially passaging a fluconazole-susceptible dose-dependent strain, YO1-16 (fluconazole MIC, 16 μg ml−1) in increasing concentrations of fluconazole, resulting in strains YO1-32 (fluconazole MIC, 32 μg
American Society for Microbiology.
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31. Topological and modulated distribution of surface markers on endothelial cells.
The mobility and distribution of angiotensin-converting enzyme (peptidyl-dipeptide hydrolase, EC 3.4.15.1) and a specific endothelial cell surface protein was assessed by fluorescein-conjugated monoclonal antibodies on bovine and murine endothelial cells grown on their extracellular matrix. The combination of data obtained from fluorescence recovery after ph
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32. Osmotic Water Permeability of Isolated Protoplasts. Modifications during Development1
A transference chamber was developed to measure the osmotic water permeability coefficient (Pos) in protoplasts 40 to 120 μm in diameter. The protoplast was held by a micropipette and submitted to a steep osmotic gradient created in the transference chamber. Pos was derived from the changes in protoplast dimensions, as measured using a light microscop
American Society of Plant Physiologists.
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33. Within-breath modulation of left ventricular function during normal breathing and positive-pressure ventilation in man.
1. To investigate how intrathoracic pressure affects left ventricular function during normal breathing and positive-pressure ventilation, beat-by-beat measurements of left ventricular stroke volume (LVSV; pulsed Doppler ultrasound), heart rate and blood pressure were made in five normal subjects breathing actively and then undergoing passive positive-pressur
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34. Localization, Purification, and Functional Reconstitution of the P4-ATPase Atp8a2, a Phosphatidylserine Flippase in Photoreceptor Disc Membranes*
P4-ATPases comprise a relatively new subfamily of P-type ATPases implicated in the energy-dependent translocation of aminophospholipids across cell membranes. In this study, we report on the localization and functional properties of Atp8a2, a member of the P4-ATPase subfamily that has not been studied previously. Reverse transcription-PCR revealed high expre
American Society for Biochemistry and Molecular Biology.
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35. Kinetics of activation of the sodium conductance in the squid giant axon.
The time course of the rise in sodium conductance during positive voltage-clamp pulses was measured in squid giant axons perfused with CsF and immersed in low-sodium solutions. The initial transients were eliminated by subtraction of records made after blocking the sodium channels with tetrodotoxin. The value of tau m as defined by Hodgkin & Huxley (1952) pa
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36. Structure, Dynamics, and Ion Conductance of the Phospholamban Pentamer
A 52-residue membrane protein, phospholamban (PLN) is an inhibitor of an adenosine-5′-triphosphate-driven calcium pump, the Ca2+-ATPase. Although the inhibition of Ca2+-ATPase involves PLN monomers, in a lipid bilayer membrane, PLN monomers form stable pentamers of unknown biological function. The recent NMR structure of a PLN pentamer depicts cytoplasmic
The Biophysical Society.