Variabilidade genética Atta robusta Borgmeier (Hymenoptera: Formicidae) com o uso de marcadores microssatélites / Genetic variability on Atta robusta Borgmeier (Hymenoptera: Formicidae) using microsatellite primers

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

22/02/2011

RESUMO

This is the first genetic study on Atta robusta to investigate the genetic variability and structure in populations from Espírito Santo and Rio de Janeiro in which microsatellites specific primers were used. Twenty microsatellite primers for A. robusta were designed and eleven (55%) were polymorphic. When analyzing 80 colonies of A. robusta, the number of alleles/locus ranged from 7 to 24. The observed heterozigosity ranged from 0,27 a 0,80 in the loci analyzed, meanwhile the expected heterozigosity ranged from 0,30 to 0,83. When grouping the colonies within the two targeted populations (Espírito Santo and Rio de Janeiro), it was confirmed that they are genetically similar. In the population from Espírito Santo, the number of alleles/locus ranged from 6 to 22. The observed heterozigosity ranged from 0,22 to 0,70, while the expected heterozygosity ranged from 0,30 to 0,85. In the population from Rio de Janeiro, the number of alleles/locus ranged from 4 to 17. The observed heterozigosity ranged from 0,32 to 0,97, while the expected ranged from 0,29 to 0,81. However, when considering the colonies sampled in each of the 16 localities as a subpopulation, it was confirmed that they are highly structured. However, the UPGMA analysis did not provide accurate evidence that the formation of clades containing only colonies are geographically nearby. When considering the 80 colonies sampled, they weren’t structured and did not group colonies geographically adjacent. These results indicate that the colonies of A. robusta sampled either in Rio de Janeiro or Espírito Santo have high genetic variability and that they aren’t structured. Although, considering the endemism and the threaten to extinction of A. robusta, in order to conserve this species will be necessary the maintenance of all possible colonies in the Restinga’s region.

ASSUNTO(S)

microssatélites variabilidade formiga cortadeira genetica animal microsatellites variability cutter ant

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