VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS
AUTOR(ES)
Anschau, A., Caruso, C. S., Kuhn, R. C., Franco, T. T.
FONTE
Braz. J. Chem. Eng.
DATA DE PUBLICAÇÃO
2017-01
RESUMO
Abstract A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is reproducible and easy to implement. A 96-well microplate SPV assay allows one to determine quickly total lipids in lyophilized cells of oleaginous yeast and microalgae. The new assay method possesses many advantages compared to the others described in the literature: requires a small amount sample, less time (around 1 h) and less labor and does not require organic reagents like chloroform in the reaction.
Documentos Relacionados
- Biodiversity of the oleaginous microorganisms in Tibetan Plateau
- A simple HPLC method for the determination of halcinonide in lipid nanoparticles: development, validation, encapsulation efficiency, and in vitro drug permeation
- Expression profile and subcellular location of the plasmid-encoded virulence (Spv) proteins in wild-type Salmonella dublin.
- A Filtration Method for the Determination of Concentration of Microorganisms in Air
- Improved Method for Determination of Respiring Individual Microorganisms in Natural Waters