TRK2 is not a low-affinity potassium transporter in Saccharomyces cerevisiae.

AUTOR(ES)

Ramos, J

RESUMO

TRK1 and TRK2 encode proteins involved in K+ uptake in Saccharomyces cerevisiae. A kinetic study of Rb+ influx in trk1 TRK2, trk1 TRK2D, and trk1 trk2 mutants reveals that TRK2 shows moderate affinity for Rb+. K(+)-starved trk1 delta TRK2 cells show a low-affinity component accounting for almost the total Vmax of the influx and a moderate-affinity component exhibiting a very low Vmax. Overexpression of TRK2 in trk1 delta TRK2D cells increases the Vmax of the moderate-affinity component, and this component disappears in trk1 delta trk2 delta cells. In contrast, the low-affinity component of Rb+ influx in trk1 delta TRK2 cells is not affected by mutations in TRK2. Consistent with the different levels of activity of the moderate-affinity Rb+ influx, trk1 delta TRK2 cells grow slowly in micromolar K+, trk1 delta TRK2D cells grow rapidly, and trk1 delta trk2 delta cells fail to grow. The existence of a unique K+ uptake system composed of several proteins is also discussed.

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