Transformation of Bacillus cereus vegetative cells by electroporation.
AUTOR(ES)
Belliveau, B H
RESUMO
Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with unpurified total plasmid DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202922Documentos Relacionados
- High-efficiency transformation of bacterial cells by electroporation.
- Transformation of Saccharomyces cerevisiae by electroporation.
- Transformation of filamentous fungi by electroporation.
- Transformation of Schizosaccharomyces pombe by electroporation.
- Transformation of Pseudomonas aeruginosa by electroporation.
