Transferability and genetic relatedness of high-level gentamicin resistance among enterococci.
AUTOR(ES)
Sahm, D F
RESUMO
Gentamicin resistance in six enterococcal species was investigated. Transfer of resistance was observed for the donors E. faecium UC 79, E. avium CC 54, and E. gallinarum B 51, but not for E. raffinosus UC 78 or E. casseliflavus UC 73. Except for E. casseliflavus UC 73, homology was observed between the E. faecalis aac6'-aph2" gene and DNA from other species. Whereas 2.6-kb HindIII fragments encoded resistance in E. faecalis UC 244, its transconjugant, and E. raffinosus UC 78, 3.4-kb fragments encoded resistance in E. faecium UC 79, E. gallinarum B 51, and their transconjugants. A 3.4-kb fragment encoded resistance in E. avium CC 54, but 2.6-kb fragments encoded resistance in the transconjugants. Although many similarities were found among the strains, the heterogeneity in gentamicin resistance exhibited by some isolates indicates diversity among these determinants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=188178Documentos Relacionados
- Use of the E test to predict high-level resistance to aminoglycosides among enterococci.
- Occurrence of high-level aminoglycoside resistance in environmental isolates of enterococci.
- Evaluation of the Vitek GPS-TA card for laboratory detection of high-level gentamicin and streptomycin resistance in enterococci.
- Investigation of the reformulated Remel Synergy Quad plate for detection of high-level aminoglycoside and vancomycin resistance among enterococci.
- Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci.