Transferability and characterization of microsatellite markers in two Neotropical Ficus species

AUTOR(ES)

Nazareno, Alison Gonçalves, Pereira, Rodrigo Augusto Santinelo, Feres, Juliana Massimino, Mestriner, Moacyr Antônio, Alzate-Marin, Ana Lilia

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

03/07/2009

RESUMO

Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.

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