Time collecting/processing and umbilical cord blood sample quality / Tempo coleta/processamento e qualidade da amostra de sangue de cordão umbilical

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Objective: To evaluate the association between the time interval from umbilical cord blood sampling until analysis and the quality of the sample. Materials and methods: Umbilical cord blood samples collected during the third stage of labor were placed in temperature-controlled boxes for the transport of biological material and sent to an umbilical cord blood bank, where the number of nucleated cells, the number of viable cells and the number of CD34+ cells were counted, and samples were additionally tested for contamination, at the following time intervals: up to 24 hours, up to 48 hours and up to 72 hours following sampling. The data were analyzed using the multivariate analysis of variance (MANOVA) and compared using McNemar s chi-square test. Significance was defined at p<0.05. Results: Means and medians of the number of nucleated cells, number of viable cells and number of CD34+ cells decreased significantly (p<0.0001) as a function of the increased time between sampling and analysis, the difference between 24 and 48 hours being less than the difference between 24 and 72 hours. A linear correlation was found between the mean number of viable cells and CD34+ cells at the three moments of analysis. Contamination testing was negative in all samples. Conclusions: The increase in the interval of time from sampling until analysis negatively affected the number of nucleated cells, viable cells and CD34+ cells but was not associated with specimen contamination. A linear correlation was found between the decrease in the number of viable cells and CD34+ cells.

ASSUNTO(S)

cordão umbilical umbilical cord stem cells fetal blood quality control adult stem cells celulas-tronco celulas-tronco adultas sangue fetal controle de qualidade

ACESSO AO ARTIGO

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