The Use of Iodinated RNA for Gene Localization

AUTOR(ES)

Prensky, Wolf

RESUMO

Carrier-free iodination of 5S RNA with 125I yields a probe suitable for use in cytological RNA-DNA hybridization studies. The genes coding for 5S ribosomal RNA in Drosophila could be localized by autoradiography after a 2-day exposure, whereas a 2-month exposure is needed when the best available [3H]RNA is used. The procedure introduces one covalently bound 125I atom per 100 nucleotides, resulting in a specific activity of over 108 dpm/μg of RNA. The method may be readily applied to other problems involving molecular hybridization techniques.

Documentos Relacionados

• Use of radio-iodinated serum albumin in the study of cerebrospinal fluid flow.
• The use of R-looping for structural gene identification and mRNA purification.
• Reversible and irreversible labeling and autoradiographic localization of the cerebral histamine H2 receptor using [125I]iodinated probes.
• A method for the determination of free 131I in radio-iodinated lipids
• Radioimmunoassay of Iodinated Tobramycin