The structural organization of dinucleosomes and oligonucleosomes. Electric dichroism and birefringence study.
AUTOR(ES)
Houssier, C
RESUMO
The spatial organization of nucleosomes and linker DNA in dinucleosomes and oligonucleosomes of various chain lengths has been investigated through electric dichroism, birefringence and relaxation times measurements at low ionic strengths (0.5 to 2.2 mM). From the negative dichroism observed for all the samples, it is concluded that the nucleosome subunits in the oligonucleosome chain must lie with their disc planes closely parallel to the fibre axis. The large increase of the negative dichroism of dinucleosomes upon Hl removal is interpreted by the unwinding of the DNA tails and the internucleosomal segment. All the samples displayed, under bipolar pulses, a predominantly induced orientation mechanism.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327559Documentos Relacionados
- Simultaneous measurement of electric birefringence and dichroism. A study on photosystem 1 particles.
- Effect of HMG protein 17 on the thermal stability of control and acetylated HeLa oligonucleosomes.
- The organization of oligonucleosomes in yeast.
- Imaging linear birefringence and dichroism in cerebral amyloid pathologies
- The internal organization of hospitals: a descriptive study.