The Sequence-Variable, Single-Copy tprK Gene of Treponema pallidum Nichols Strain UNC and Street Strain 14 Encodes Heterogeneous TprK Proteins
AUTOR(ES)
Stamm, Lola V.
FONTE
American Society for Microbiology
RESUMO
Syphilis is a chronic infection with early relapses that are hypothesized to result from the emergence of phenotypic variants of Treponema pallidum. Recent studies demonstrated that TprK, a target of protective immunity, is heterogeneous in several T. pallidum strains, but not in Nichols strain Seattle (A. Centurion-Lara, C. Godornes, C. Castro, W. C. Van Voorhis, and S. A. Lukehart, Infect. Immun. 68:824–831, 2000). Analysis of PCR-amplified tprK from Nichols strain UNC and Street strain 14 treponemes showed that TprK has seven regions of intrastrain heterogeneity resulting from amino acid substitutions, insertions, and deletions. In contrast, analysis of PCR-amplified tprJ showed little intrastrain or interstrain heterogeneity. Reverse transcriptase PCR analysis demonstrated that mRNA transcripts representing unique polymorphic TprK proteins are present during syphilitic infection. Southern hybridization confirmed that Nichols strain UNC and Street strain 14 each contain a single copy of tprK, indicating that intrastrain heterogeneity is due to the presence of multiple treponemal subpopulations which contain a variant form of tprK.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=97738Documentos Relacionados
- The tprK Gene Is Heterogeneous among Treponema pallidum Strains and Has Multiple Alleles
- Sequence Diversity of Treponema pallidum subsp. pallidum tprK in Human Syphilis Lesions and Rabbit-Propagated Isolates
- A Single-Copy Gene Encodes Kex1, a Serine Endoprotease of Pneumocystis jiroveci
- Characterization and evolution of a single-copy sequence from the human Y chromosome.
- Distinct single-copy sequence sets in sea urchin nuclear RNAs.