The origin of bidirectional DNA replication in polyoma virus.
AUTOR(ES)
Hendrickson, E A
RESUMO
The nucleotide locations of RNA-p-DNA covalent linkages in polyoma virus (PyV) replicating DNA were mapped in the region containing the genetically required origin of DNA replication (ori). These linkages mark the initiation sites for RNA-primed DNA synthesis. A clear transition was identified between the presence of these linkages (discontinuous DNA synthesis) and their absence (continuous DNA synthesis) on each strand of ori. This demonstrated that PyV DNA replication, like simian virus 40 (SV40), is semi-discontinuous, and thus revealed the location of the origin of bidirectional DNA replication (OBR). The transition site on the template encoding PyV late mRNA occurred at the junction of ori-core and T-antigen binding site A. This was essentially the same site as previously observed in SV40 (Hay and DePamphilis, 1982). However, in contrast to SV40, the transition site on the template encoding PyV early mRNA was displaced towards the late gene side of ori. This resulted in a 16 nucleotide gap within ori in which no RNA-p-DNA linkages were observed on either strand. A model for the initiation of PyV DNA replication is presented.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553590Documentos Relacionados
- Asynchronous bidirectional replication of polyoma virus DNA.
- DNA replication origin of polyoma virus: early proximal boundary.
- Species-specific in vitro synthesis of DNA containing the polyoma virus origin of replication.
- Similarity of nucleotide sequences around the origin of DNA replication in mouse polyoma virus and simian virus 40.
- cis-acting elements in the lytic origin of DNA replication of Epstein-Barr virus.