The first Canadian indigenous case of bovine spongiform encephalopathy (BSE) has molecular characteristics for prion protein that are similar to those of BSE in the United Kingdom but differ from those of chronic wasting disease in captive elk and deer
AUTOR(ES)
Stack, Michael J.
FONTE
Canadian Veterinary Medical Association
RESUMO
Brain tissue from a case of bovine spongiform encephalopathy (BSE) from Alberta was subjected to a Western immunoblotting technique to ascertain the molecular profile of any disease-specific, abnormal prion protein, that is, prion protein that is protease-resistant (PrPres). This technique can discriminate between isolates from BSE, ovine scrapie, and sheep experimentally infected with BSE. Isolates of brain tissue from the BSE case in Alberta, 3 farmed elk with chronic wasting disease (CWD) from different parts of Saskatchewan, and 1 farmed white-tailed deer with CWD from Edmonton, Alberta, were examined alongside isolates of brain tissue from BSE, ovine scrapie, and sheep experimentally infected with BSE from the United Kingdom (UK). The molecular weights of PrPres and the cross reactions to 2 specific monoclonal antibodies (mAbs) were determined for each sample. The BSE isolates from Canada and the UK had very similar PrPres molecular weights and reacted with only 1 of the 2 mAbs. The PrPres isolated from both elk and white-tailed deer with CWD had a higher molecular weight profile than did the corresponding PrPres from the scrapie and BSE isolates. The PrPres from CWD cases cross reacted with both mAbs, a property shared with PrPres in isolates from scrapie but not with PrPres isolates from BSE or sheep experimentally infected with BSE. The results from this study seem to confirm that the PrPres isolated from the BSE case in Alberta has similar molecular properties to the PrPres isolated from a BSE case in the UK, and that it differs in its molecular and immunological characteristics from the CWD and scrapie cases studied.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=545986Documentos Relacionados
- EMBRYO TRANSFER AND BOVINE SPONGIFORM ENCEPHALOPATHY (BSE)
- Molecular Analysis of Cases of Italian Sheep Scrapie and Comparison with Cases of Bovine Spongiform Encephalopathy (BSE) and Experimental BSE in Sheep
- Comparison of Abnormal Prion Protein Glycoform Patterns from Transmissible Spongiform Encephalopathy Agent-Infected Deer, Elk, Sheep, and Cattle
- Population-Level Retrospective Study of Neurologically Expressed Disorders in Ruminants before the Onset of Bovine Spongiform Encephalopathy (BSE) in Belgium, a BSE Risk III Country
- Oral infection by the bovine spongiform encephalopathy prion