The exon–exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay
AUTOR(ES)
Le Hir, Hervé
FONTE
Oxford University Press
RESUMO
We recently reported that spliceosomes alter messenger ribonucleoprotein particle (mRNP) composition by depositing several proteins 20–24 nucleotides upstream of mRNA exon–exon junctions. When assembled in vitro, this so-called ‘exon–exon junction complex’ (EJC) contains at least five proteins: SRm160, DEK, RNPS1, Y14 and REF. To better investigate its functional attributes, we now describe a method for generating spliced mRNAs both in vitro and in vivo that either do or do not carry the EJC. Analysis of these mRNAs in Xenopus laevis oocytes revealed that this complex is the species responsible for enhancing nucleocytoplasmic export of spliced mRNAs. It does so by providing a strong binding site for the mRNA export factors REF and TAP/p15. Moreover, by serving as an anchoring point for the factors Upf2 and Upf3, the EJC provides a direct link between splicing and nonsense-mediated mRNA decay. Finally, we show that the composition of the EJC is dynamic in vivo and is subject to significant evolution upon mRNA export to the cytoplasm.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=125616Documentos Relacionados
- Splicing of U12-type introns deposits an exon junction complex competent to induce nonsense-mediated mRNA decay
- Nonsense-Mediated Decay of Human HEXA mRNA
- Identification and characterization of a sequence motif involved in nonsense-mediated mRNA decay.
- Nonsense-Mediated Decay of Mutant waxy mRNA in Rice
- Characterization of cis-acting sequences and decay intermediates involved in nonsense-mediated mRNA turnover.