The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
AUTOR(ES)
Mansouri, Vahid, Salehi, Mohammad, Nourozian, Mohsen, Fadaei, Fatemeh, Farahani, Reza Mastery, Piryaei, Abbas, Delbari, Ali
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
01/05/2015
RESUMO
Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes related to different stages of PGC development. Flow cytometry and immunocytochemical staining confirmed the presence of Mvh protein in a small number of cells. There were significant differences between cells that differentiated into PGCs in the group treated with Bmp4 compared to non-treated cells. These findings indicate that ntESCs can differentiate into putative PGCs. Improvement of ntESC differentiation into PGCs may be a reliable means of producing mature germ cells.
Documentos Relacionados
- Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology
- Derivation of pluripotent stem cells from cultured human primordial germ cells
- Dissection of the c-Kit signaling pathway in mouse primordial germ cells by retroviral-mediated gene transfer
- Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes
- Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation