Teste rápido TR-DPP no contexto do diagnóstico sorológico da leishmaniose visceral canina / Rapid test TR-DPP in the context of serological diagnosis of canine visceral leishmaniasis

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

22/05/2012

RESUMO

American visceral leishmaniasis caused by Leishmania (L.) infantum chagasi represents a serious public health problem in Brazil, with endemic character and on expansion. Transmission occurs by female phlebotomine sandflies. The dog, considered the domestic reservoir of the disease plays an important role due to the parasitism of skin and proximity to the man. One of the disease control measures adopted by the Ministry of Health is the serological detection of infected animals and their subsequent elimination. The current diagnostic protocol uses the ELISA-Bio-Manguinhos as test for screening and IFIs-Bio- Manguinhos as confirmatory. Among the problems cited for the serological diagnosis of visceral leishmaniasis are the low sensitivity in asymptomatic animals and cross reactions mainly with Ehrlichia canis. New technologies have emerged for the diagnosis of CVL, among them the quick test Dual Path Platform (DPP-TR -Bio-Manguinhos), that from 2012 on will be part of the official protocol for diagnosis of the disease, as screening and ELISA- Bio-Manguinhos as confirmatory test. The objectives of the first study were to assess the sensitivity of tests (IFI, ELISA and TR-DPP ) alone, and to compare the existing diagnostic protocol (ELISA followed by IFI) with the proposed one (TR-DPP followed by ELISA) in samples of asymptomatic dogs with positive parasitological examination. It was concluded that IFI has identified the largest number of infected dogs, followed by ELISA and TR-DPP which had equivalent results, and that the performance of the proposed protocol did not provide any significant difference in number of animals diagnosed in relation to the existing protocol, with a good accordance between them regarding Kappa classification. The aim of the second study was to determine the possible influence of infection with Ehrlichia canis in TR-DPP and it was concluded by means of statistical analysis that there is no association between presenting Ehrlichia canis morulae and a positive serological reaction in TR-DPP test. The PCR of the genus leishmania held in TR-DPP positive samples that showed negative parasitological results but with the presence of morulae confirmed the lack of association between the presence of morulae and the positive result in TR-DPD.

ASSUNTO(S)

diagnóstico leishmania cães bactérias gram-negativas doencas infecciosas e parasitarias diagnosis dogs gram-negative bacteria

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