Study of the effects of naphthalene and benzo(a) pyrene in Trachinotus carolinus (Perciformes, Carangidae) using citogenotoxic, histopahological and biochemical biomarkers. / Estudo sobre efeitos do naftaleno e benzo(a) pireno em Trachinotus carolinus (Perciformes, Carangidae) utilizando biomarcadores citogenotóxicos, histopatológicos e bioquímicos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Effects of exposure of fish to pollutants can be identified through stress responses. The present study aims to evaluate the effects of naphthalene and benzo(a)pyrene in Florida pompanos, Trachinotus carolinus. Evidences from citogenotoxical, histopathological and biochemical studies showed that alterations caused by exposures to 0.9 M, 2.7 M and 8.1 M of NAP and BAP occurred within 12 to 96 hours. NAP at 8.1 M induced erythrocyte DNA strand breaks in pompanos since early periods of exposure. Genotoxic effects of BAP at the lowest concentration were documented soon after 24 hours of exposure. Mutagenotoxicity of both pollutants, as seen by the induction of MN and ENA, was revealed since early periods and their frequencies are related to the duration of exposure. Exposures to these PAHs, for longer periods, resulted in increased frequency and severity of lesions observed in fish tissues. Specificity of CYP1A, observed through immunohistochemical analyses, was related to the dose of the pollutants and mainly at longer periods of exposure. These organic pollutants, under the experimental conditions, did not interfere with the activity of liver catalase and GST of the species. The citogenotoxic and histopathologic biomarkers used in this study proved to be efficient tools to ascertain the toxicity, genotoxicity and mutagenesis of NAP and BAP, as well as their dose related response, in the species T. carolinus.

ASSUNTO(S)

catalase trachinotus carolinus histopatologia glutationa- s-transferase naftaleno genotoxicity catalase benzo(a)pyrene trachinotus carolinus genotoxicidade cyp1a histopathology cyp1a glutathione-stransferase benzo(a)pireno naphthalene

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