Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus
AUTOR(ES)
Cheng, S. W., Wang, Y. F., Hong, B.
FONTE
Braz. J. Chem. Eng.
DATA DE PUBLICAÇÃO
2012-12
RESUMO
The nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH4)2SO4, 8 g/L urea, 1 g/L NH4Cl, 3 g/L KH2PO4, 1 g/L FeSO4·7H2O, 2 g/L ZnSO4·7H2O, 2 g/L CaCl2·6H2O, 2 g/L MnSO4·H2O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium.
Documentos Relacionados
- Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells
- Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
- Medium composition influence on Biotin and Riboflavin production by newly isolated Candida sp
- Streptomyces albus Isolated from a Human Actinomycetoma and Characterized by Molecular Techniques
- Production, purification, and characterization of an extracellular chitosanase from Streptomyces.