Simple, rapid, and quantitative release of periplasmic proteins by chloroform.

AUTOR(ES)

Ames, G F

RESUMO

We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.

Documentos Relacionados

• A Simple, Rapid, and Reliable Titrimetric Method for the Determination of Glycerol at Low Concentration
• Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method
• A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences
• Inactivation of hepatitis B virus and non-A, non-B hepatitis by chloroform.
• Rapid, Simple Enzyme Immunoassay for Gentamicin