Seqüenciamento de peptídeos usando espectrometria de massas: um guia prático

AUTOR(ES)
FONTE

Química Nova

DATA DE PUBLICAÇÃO

2008

RESUMO

This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing. The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience. In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.

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