Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthase proteolipid subunit.

AUTOR(ES)

Young, E G

RESUMO

We have sequenced the Petunia hybrida gene that specifies the proteolipid subunit of the mitochondrial Fo ATP synthase and have used this gene to investigate plant mitochondrial gene transcription. The Petunia atp 9 gene contains a single open-reading frame capable of specifying a 77 amino acid-polypeptide that is homologous to bovine, fungal and maize proteolipid subunits. S1 protection identified 3 transcripts in a ratio of 1:5:100 in the Petunia tissues tested. The transcripts share a common 3' terminus but have 5' termini that map 528, 266, and 121 nucleotides upstream of the translation start site. The 5' terminus of the longest transcript maps to the sequence ATATAGTA, which is nearly identical to the yeast mitochondrial transcription initiation site ATATAAGTA. Primer extension analysis indicates that these two shorter transcripts are not due to splicing. The two shorter transcripts originate at sequences homologous to sites at 5' termini of two pea and maize genes. These consensus sequences may signal processing events other than splicing.

Documentos Relacionados

• A Functional Mitochondrial Atp Synthase Proteolipid Gene Produced by Recombination of Parental Genes in a Petunia Somatic Hybrid
• Specific mitochondrial proteins in pollen: presence of an additional ATP synthase beta subunit.
• Numerous transcription initiation sites exist for the maize mitochondrial genes for subunit 9 of the ATP synthase and subunit 3 of cytochrome oxidase.
• The imported preprotein of the proteolipid subunit of the mitochondrial ATP synthase from Neurospora crassa. Molecular cloning and sequencing of the mRNA.
• Location and nucleotide sequence of the gene for the proton-translocating subunit of wheat chloroplast ATP synthase