Sensitive Method for Detection of Human Herpesviruses 6 and 7 in Saliva Collected in Field Studies

AUTOR(ES)
FONTE

American Society for Microbiology

RESUMO

To facilitate studies of the epidemiology and natural history of human herpesviruses 6 and 7 in infants, a practical method for collecting and quantifying the DNA of these viruses was developed. Saliva was collected using small strips of filter paper, and virus was detected using a real-time quantitative fluorescent-probe PCR assay. The sensitivity and specificity of this method even after prolonged drying of the specimens compared favorably to those of our traditional method of collecting and assaying saliva.

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