Role of ctc from Listeria monocytogenes in Osmotolerance
AUTOR(ES)
Gardan, Rozenn
FONTE
American Society for Microbiology
RESUMO
Listeria monocytogenes is a food-borne pathogen with the ability to grow under conditions of high osmolarity. In a previous study, we reported the identification of 12 proteins showing high induction after salt stress. One of these proteins is highly similar to the general stress protein Ctc of Bacillus subtilis. In this study, induction of Ctc after salt stress was confirmed at the transcriptional level by using RNA slot blot experiments. To explore the role of the ctc gene product in resistance to stresses, we constructed a ctc insertional mutant. No difference in growth was observed between the wild-type strain LO28 and the ctc mutant either in rich medium after osmotic or heat stress or in minimal medium after heat stress. However, in minimal medium after osmotic stress, the growth rate of the mutant was increased by a factor of 2. Moreover, electron microscopy analysis showed impaired morphology of the mutant grown under osmotic stress conditions in minimal medium. Addition of the osmoprotectant glycine betaine to the medium completely abolished the osmotic sensitivity phenotype of the ctc mutant. Altogether, these results suggest that the Ctc protein of L. monocytogenes is involved in osmotic stress tolerance in the absence of any osmoprotectant in the medium.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=152465Documentos Relacionados
- A Postgenomic Appraisal of Osmotolerance in Listeria monocytogenes
- Cloning of rel from Listeria monocytogenes as an Osmotolerance Involvement Gene
- Identification of the Gene Encoding the Alternative Sigma Factor ςB from Listeria monocytogenes and Its Role in Osmotolerance†
- Glycine betaine confers enhanced osmotolerance and cryotolerance on Listeria monocytogenes.
- Mutational Analysis of the Role of HPr in Listeria monocytogenes†