RNAi-induced silencing of embryonic tryptophan oxygenase in the Pyralid moth, Plodia interpunctella
AUTOR(ES)
Fabrick, Jeffrey A.
FONTE
University of Arizona Library
RESUMO
Gene silencing through the introduction of double-stranded RNA (RNA interference, RNAi) provides a powerful tool for the elucidation of gene function in many systems, including those where genomics and proteomics are incomplete. The use of RNAi technology for gene silencing in Lepidoptera has lacked significant attention compared to other systems. To demonstrate that RNAi can be utilized in the lepidopteran, Plodia interpunctella, we cloned a cDNA for tryptophan oxygenase, and showed that silencing of tryptophan oxygenase through RNAi during embryonic development resulted in loss of eye-color pigmentation. The complete amino acid sequence of Plodia tryptophan oxygenase can be accessed through NCBI Protein Database under NCBI Accession # AY427951.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=528875Documentos Relacionados
- Toxicity of parasporal crystals of Bacillus thuringiensis to the Indian meal moth, Plodia interpunctella.
- Isolation and purification of a granulosis virus from infected larvae of the Indian meal moth, Plodia interpunctella.
- Inhibitors of calling behavior of Plodia interpunctella
- Structural Polypeptides of the Granulosis Virus of Plodia interpunctella†
- Two-Dimensional Polyacrylamide Gel Analysis of Plodia interpunctella Granulosis Virus †
